The present study describes a novel yeast model for the prediction of yeast fermentation. The proposed model considers the possible metabolic pathways of yeast. For each pathway, the time evolution of components, energy (ATP/ADP), and electron carriers (NAD+ /NADH) are expressed with limitation factors for all quantities consumed by each respective pathway. In this manner, the model can predict the partition of these pathways based on the growth conditions and their evolution over time. Several biological pathways and their stoichiometric coefficients are well known from literature. It is important to note that most of the kinetic parameters have no effect as the actual kinetics are controlled by the balance of limiting factors. The few remaining parameters were adjusted and compared with the literature when the data set was available. The model fits our experimental data from yeast fermentation on glucose in a nonaerated batch system. The predictive ability of the model and its capacity to represent the intensity of each pathway over time facilitate an improved understanding of the interactions between the pathways. The key role of energy (ATP) and electron carrier (NAD+ ) to trigger the different metabolic pathways during yeast growth is highlighted, whereas the involvement of mitochondrial respiration not being associated with the TCA cycle is also shown.
Keywords: TCA cycle; energy balance; fermentation; metabolic pathway; respiration.
© 2020 Wiley Periodicals LLC.