Fully automated, low-cost ion chromatography system for in-situ analysis of nitrite and nitrate in natural waters

Eoin Murray; Patrick Roche; Matthieu Briet; Breda Moore; Aoife Morrin; Dermot Diamond; Brett Paull

doi:10.1016/j.talanta.2020.120955

Fully automated, low-cost ion chromatography system for in-situ analysis of nitrite and nitrate in natural waters

Talanta. 2020 Aug 15:216:120955. doi: 10.1016/j.talanta.2020.120955. Epub 2020 Mar 19.

Authors

Eoin Murray¹, Patrick Roche², Matthieu Briet², Breda Moore², Aoife Morrin³, Dermot Diamond³, Brett Paull⁴

Affiliations

¹ Research & Development, T.E. Laboratories Ltd. (TelLab), Tullow, Carlow, Ireland; Insight Centre for Data Analytics, National Centre for Sensor Research, School of Chemical Sciences, Dublin City University, Dublin 9, Ireland.
² Research & Development, T.E. Laboratories Ltd. (TelLab), Tullow, Carlow, Ireland.
³ Insight Centre for Data Analytics, National Centre for Sensor Research, School of Chemical Sciences, Dublin City University, Dublin 9, Ireland.
⁴ Australian Centre for Research on Separation Science (ACROSS), School of Physical Sciences, University of Tasmania, Sandy Bay, Hobart, 7001, Australia; ARC Training Centre for Portable Analytical Separation Technologies (ASTech), School of Physical Sciences, University of Tasmania, Sandy Bay, Hobart, 7001, Australia. Electronic address: Brett.Paull@utas.edu.au.

PMID: 32456896
DOI: 10.1016/j.talanta.2020.120955

Abstract

A cost-effective, automated and portable IC has been developed for in-situ analysis of nitrite and nitrate in natural waters. The system employed 3D printed pumps for eluent delivery and a deep-UV LED based optical detector. Isocratic separation and selective detection of nitrite and nitrate was achieved in under 3 min. The total weight of the analyser was ~11 kg, and included electronics along with a sample intake system for automated analysis. Linear calibration ranges were generated using different sample injection loops. Using a 150 μL loop, an analytical range (0.05-30 mg L^-1 NO₂^-, 0.10-75 mg L^-1 NO₃^-) suitable for freshwater analysis was generated, while using a 10 μL loop an analytical range (0.30-100 mg L^-1 NO₂^-, 2.5-500 mg L^-1 NO₃^-) suitable for effluent and domestic wastewater analysis was achieved. Chromatographic repeatability demonstrated by the system is graphically presented and RSD values of <4% were obtained in terms of peak area and retention time over 82 sequential runs. The system was deployed in-situ at multiple sites for varying deployment periods analysing septic tank water, effluent from a waste water treatment plant and stream water. The data generated by the in-situ system were comparable to grab sample data generated by accredited laboratory instrumentation.

Keywords: Ion chromatography; Nitrate; Nutrients; Portable; UV light-emitting diode; Water analysis.