Comparison of biochemical parameters and chemokine levels in pleural fluid between patients with anergic and non-anergic tuberculous pleural effusion

Ha-Jeong Kim; Suyeon Ryu; Sun Ha Choi; Hyewon Seo; Seung Soo Yoo; Shin Yup Lee; Seung Ick Cha; Jae Yong Park; Chang Ho Kim; Jaehee Lee

doi:10.1016/j.tube.2020.101940

Comparison of biochemical parameters and chemokine levels in pleural fluid between patients with anergic and non-anergic tuberculous pleural effusion

Tuberculosis (Edinb). 2020 Jul:123:101940. doi: 10.1016/j.tube.2020.101940. Epub 2020 May 13.

Authors

Affiliations

¹ Department of Physiology, Cell and Matrix Research Institute, BK21 Plus KNU Biomedical Convergence Program, Tumor Heterogeneity and Network (THEN) Research Center, Kyungpook National University, School of Medicine, Daegu, South Korea; Kyungpook National University Bio-Medical Research Institute, Daegu, South Korea.
² Department of Physiology, Cell and Matrix Research Institute, BK21 Plus KNU Biomedical Convergence Program, Tumor Heterogeneity and Network (THEN) Research Center, Kyungpook National University, School of Medicine, Daegu, South Korea.
³ Department of Internal Medicine, Kyungpook National University, School of Medicine, Daegu, South Korea.
⁴ Department of Internal Medicine, Kyungpook National University, School of Medicine, Daegu, South Korea. Electronic address: kimch@knu.ac.kr.
⁵ Department of Internal Medicine, Kyungpook National University, School of Medicine, Daegu, South Korea; Kyungpook National University Bio-Medical Research Institute, Daegu, South Korea. Electronic address: jaelee@knu.ac.kr.

PMID: 32452425
DOI: 10.1016/j.tube.2020.101940

Abstract

Pleural fluid (PF) immune response in anergic tuberculous pleural effusion (TPE) patients is poorly understood. This study aimed to compare PF biochemical parameters and chemokine levels between anergic and non-anergic TPE patients. Chemokine arrays, cytokine measurements, and flow cytometry were performed in 58 patients (TPE [non-anergic (n = 32) and anergic (n = 10)] and malignant pleural effusion (MPE) [n = 16]). PF adenosine deaminase 2 (ADA2) levels were significantly lower in anergic TPE patients than in non-anergic TPE patients (p = 0.048). Among the 40 chemokines tested, PF CCL27 levels were significantly higher in anergic TPE patients than in non-anergic TPE and MPE patients (p < 0.001). The percentage of CD4⁺CCR10⁺T cells in PF was higher in anergic TPE patients than in non-anergic TPE and MPE patients (p = 0.001). We reported here that CCL27/CCR10 interactions might contribute to pathophysiology in anergic TPE. PF CCL27 and CD4⁺CCR10⁺T cells may help in diagnosing TPE in patients with moderate elevation of PF ADA levels.

Keywords: Adenosine deaminase; Anergy; CCL27; CCR10; Tuberculous pleural effusion.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Deaminase / analysis*
Adult
Aged
Aged, 80 and over
Biomarkers / analysis
CD4 Lymphocyte Count
CD4-Positive T-Lymphocytes / immunology
Chemokine CCL27 / analysis*
Female
Flow Cytometry
Humans
Intercellular Signaling Peptides and Proteins / analysis*
Male
Middle Aged
Pleural Effusion / diagnosis
Pleural Effusion / immunology*
Pleural Effusion / microbiology
Predictive Value of Tests
Prospective Studies
Protein Array Analysis
Receptors, CCR10 / analysis
Tuberculosis, Pleural / diagnosis
Tuberculosis, Pleural / immunology*
Tuberculosis, Pleural / microbiology

Substances

Biomarkers
CCL27 protein, human
CCR10 protein, human
Chemokine CCL27
Intercellular Signaling Peptides and Proteins
Receptors, CCR10
ADA2 protein, human
Adenosine Deaminase