Identification of a novel protective antigen, 3-oxoacyl-[acyl-carrier-protein] synthase II of Streptococcus equi ssp. zooepidemicus which confers protective effects

Li Yi; Weiping Yang; Liyun Sun; Jinpeng Li; Xiaokang Li; Yang Wang

doi:10.1016/j.cimid.2020.101493

Identification of a novel protective antigen, 3-oxoacyl-[acyl-carrier-protein] synthase II of Streptococcus equi ssp. zooepidemicus which confers protective effects

Comp Immunol Microbiol Infect Dis. 2020 May 19:71:101493. doi: 10.1016/j.cimid.2020.101493. Online ahead of print.

Authors

Li Yi¹, Weiping Yang², Liyun Sun³, Jinpeng Li³, Xiaokang Li³, Yang Wang⁴

Affiliations

¹ College of Life Science, Luoyang Normal University, Luoyang, China; Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang, China.
² College of Life Science, Luoyang Normal University, Luoyang, China.
³ Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang, China; College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China.
⁴ Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang, China; College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China. Electronic address: wangyocean@163.com.

PMID: 32447155
DOI: 10.1016/j.cimid.2020.101493

Abstract

Streptococcus equi ssp. zooepidemicus (SEZ) is an important swine pathogen and responsible for a wide variety of infections in many animal species. FabF was a novel protein identified in the previous study. However, its protective efficacy remained to be evaluated. In this study, recombinant fabF of SEZ was expressed and showed a strong immunoreactivity with mini-pig convalescent sera. Study in mice revealed that the recombinant protein induced a marked antibody response and protected 80% of mice against SEZ infection. The hyperimmune sera against fabF could efficiently kill the bacteria in the phagocytosis test. In addition, it was also found that anti- fabF antibodies can significantly inhibit the formation of SEZ biofilm. These study suggest that fabF may represent immunogens of interest for vaccine development against SEZ infection.

Keywords: Immune protection; Immunogen; Streptococcus equi ssp. zooepidemicus; Vaccine.