Establishment of homozygous knock-out sea urchins

Shunsuke Yaguchi; Junko Yaguchi; Haruka Suzuki; Sonoko Kinjo; Masato Kiyomoto; Kazuho Ikeo; Takashi Yamamoto

doi:10.1016/j.cub.2020.03.057

Establishment of homozygous knock-out sea urchins

Curr Biol. 2020 May 18;30(10):R427-R429. doi: 10.1016/j.cub.2020.03.057.

Authors

Shunsuke Yaguchi¹, Junko Yaguchi², Haruka Suzuki², Sonoko Kinjo³, Masato Kiyomoto⁴, Kazuho Ikeo³, Takashi Yamamoto⁵

Affiliations

¹ Shimoda Marine Research Center, University of Tsukuba, 5-10-1 Shimoda, Shizuoka 415-0025, Japan; PRESTO, JST, 4-1-8 Honcho, Kawaguchi, 332-0012 Japan. Electronic address: yag@shimoda.tsukuba.ac.jp.
² Shimoda Marine Research Center, University of Tsukuba, 5-10-1 Shimoda, Shizuoka 415-0025, Japan.
³ Center for Information Biology, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411-8540, Japan.
⁴ Marine and Coastal Research Center, Ochanomizu University, Kou-yatsu 11, Tateyama, Chiba 294-0301, Japan.
⁵ Division of Integrated Sciences for Life, Graduate School of Integrated Sciences for Life, Hiroshima University, 1-3-1, Kagamiyama, Higashi-Hiroshima 739-8526, Japan.

PMID: 32428469
DOI: 10.1016/j.cub.2020.03.057

Abstract

Yaguchi et al. establish a homozygous knock-out sea urchin line by applying the CRISPR-Cas9 system to a new model species, Temnopleurus reevesii, whose breeding cycle takes about half a year.

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
CRISPR-Cas Systems*
DNA / chemistry
DNA / genetics
Female
Gene Expression Regulation, Enzymologic
Gene Knockout Techniques / methods*
Homozygote
Male
Mutation
Polyketide Synthases / genetics*
Sea Urchins / genetics*

Substances

Polyketide Synthases
DNA