Effect of Eriodictyol on Collagen-Induced Arthritis in Rats by Akt/HIF-1α Pathway

ZhongHua Lei; Liu Ouyang; Yong Gong; ZhaoZhen Wang; Bo Yu

doi:10.2147/DDDT.S239662

Effect of Eriodictyol on Collagen-Induced Arthritis in Rats by Akt/HIF-1α Pathway

Drug Des Devel Ther. 2020 Apr 29:14:1633-1639. doi: 10.2147/DDDT.S239662. eCollection 2020.

Authors

ZhongHua Lei^#^{1

2}, Liu Ouyang^#³, Yong Gong¹, ZhaoZhen Wang¹, Bo Yu¹

Affiliations

¹ Department of Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, People's Republic of China.
² Department of Orthopedics, The Sixth Peoples Hospital of Huizhou, Huizhou 516211, People's Republic of China.
³ Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People's Republic of China.

^# Contributed equally.

Abstract

Purpose: The aim of the experiment was to explore the effect of eriodictyol (ERI) on arthritis.

Methods: We established a rat model of collagen-induced rheumatoid arthritis (CIA) using type II collagen plus Freund's complete adjuvant. We evaluated the degree of paw swelling, joint pathology, inflammatory cytokine levels, and the Akt/hypoxia-inducible factor (HIF)-1α signaling pathway in the CIA rats.

Results: ERI significantly ameliorated joint swelling; improved joint pathology; and suppressed the release of interleukin-6, interleukin-1 beta, and tumor necrosis factor-alpha. Moreover, ERI inhibited the Akt/HIF-1α pathway in the joints of rats and in lipopolysaccharide-treated RAW264.7 cells.

Conclusion: ERI ameliorated arthritis in a manner involving the Akt/HIF-1α signaling pathway.

Keywords: Akt/HIF-1α pathway; Eriodictyol; arthritis; inflammation.

MeSH terms

Animals
Anti-Inflammatory Agents, Non-Steroidal / administration & dosage
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Arthritis, Experimental / chemically induced
Arthritis, Experimental / drug therapy*
Arthritis, Experimental / pathology
Arthritis, Rheumatoid / chemically induced
Arthritis, Rheumatoid / drug therapy*
Arthritis, Rheumatoid / pathology
Collagen / administration & dosage
Cytokines / analysis
Dose-Response Relationship, Drug
Flavanones / administration & dosage
Flavanones / pharmacology*
Hypoxia-Inducible Factor 1, alpha Subunit / antagonists & inhibitors*
Hypoxia-Inducible Factor 1, alpha Subunit / metabolism
Injections, Subcutaneous
Mice
Proto-Oncogene Proteins c-akt / antagonists & inhibitors*
Proto-Oncogene Proteins c-akt / metabolism
RAW 264.7 Cells
Rats
Rats, Sprague-Dawley
Signal Transduction / drug effects
Structure-Activity Relationship

Substances

Anti-Inflammatory Agents, Non-Steroidal
Cytokines
Flavanones
Hif1a protein, rat
Hypoxia-Inducible Factor 1, alpha Subunit
Collagen
Proto-Oncogene Proteins c-akt
eriodictyol