Control of microenvironment around enzymes by hydrogels

Yuichiro Kobayashi; Kenji Kohara; Yusuke Kiuchi; Hiroki Onoda; Osami Shoji; Hiroyasu Yamaguchi

doi:10.1039/d0cc01332c

Control of microenvironment around enzymes by hydrogels

Chem Commun (Camb). 2020 Jun 18;56(49):6723-6726. doi: 10.1039/d0cc01332c.

Authors

Yuichiro Kobayashi¹, Kenji Kohara¹, Yusuke Kiuchi¹, Hiroki Onoda², Osami Shoji², Hiroyasu Yamaguchi¹

Affiliations

¹ Department of Macromolecular Science, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan. hiroyasu@chem.sci.osaka-u.ac.jp.
² Department of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-0802, Japan. shoji.osami@a.mbox.nagoya-u.ac.jp.

PMID: 32421111
DOI: 10.1039/d0cc01332c

Abstract

We prepared enzyme-immobilized hydrogels and investigated the effects of the cross-linking density and polymer properties on their oxidation reaction rate. The oxidation rate of enzyme-immobilized hydrogels increased as the cross-linking density in the hydrogels increased. In addition, we controlled the oxidation rate using hydrogels exhibiting an appropriate interaction with a decoy molecule in the hydrogel.

MeSH terms

Cross-Linking Reagents / chemistry
Cross-Linking Reagents / metabolism*
Cytochrome P-450 Enzyme System / chemistry
Cytochrome P-450 Enzyme System / metabolism*
Enzymes, Immobilized / chemistry
Enzymes, Immobilized / metabolism
Hydrogels / chemistry
Hydrogels / metabolism*
Molecular Structure
Oxidation-Reduction
Polymers / chemistry
Polymers / metabolism*
Sphingomonas / enzymology

Substances

Cross-Linking Reagents
Enzymes, Immobilized
Hydrogels
Polymers
Cytochrome P-450 Enzyme System

Supplementary concepts

Sphingomonas paucimobilis