Tea extract increases cell fusion via regulation of cell surface DC-STAMP

Kenji Kuriya; Masahiro Nishio; Tomoko Matsuda; Hayato Umekawa

doi:10.1016/j.bbrep.2020.100759

Tea extract increases cell fusion via regulation of cell surface DC-STAMP

Biochem Biophys Rep. 2020 May 11:22:100759. doi: 10.1016/j.bbrep.2020.100759. eCollection 2020 Jul.

Authors

Kenji Kuriya¹, Masahiro Nishio¹, Tomoko Matsuda¹, Hayato Umekawa¹

Affiliation

¹ Department of Life Sciences, Graduate School of Bioresources, Mie University, 1577, Kurimamachiya, Tsu, Mie, 514-8507, Japan.

Abstract

Mononuclear osteoclast precursor cells fuse with each other to become mature multinucleated osteoclasts, which is regulated by dendritic cell-specific transmembrane protein (DC-STAMP). We evaluated the effects of tea extract and catechins on cell-cell fusion and DC-STAMP expression to elucidate their relationship with osteoclast development. When tea extract or epigallocatechin gallate (EGCg) was applied to RAW264.7 cells, multinucleated cells were increased significantly, while tartrate-resistant acid phosphatase (TRAP) activity was hardly upregulated. Flow cytometric analysis revealed that EGCg suppressed DC-STAMP expression on the cell surface, which is similar to osteoclast development. These observations suggest that TRAP activity is not activated even when suppression of both surface DC-STAMP expression and multinucleation occurs, which might be mediated by another pathway.

Keywords: Catechin; DC-STAMP; DC-STAMP, dendritic cell-specific transmembrane protein; EC, (−)-epicatechin; ECg, (−)-epicatechin gallate; EGC, (−)-epigallocatechin; EGCg, (−)-epigallocatechin gallate; Epigallocatechin gallate; RAW264.7; TEx, tea extract; Tea extract.