Three previously undescribed ent-abietane-type diterpenoids, designated as 11β-hydroxy-14-oxo-17-al-ent-abieta-8(9),13(15)-dien-16,12β-olide (1), 11β,17-dihydroxy-12-methoxy-ent-abieta-8(14),13(15)-dien-16,12ɑ-olide (2), and 14ɑ-hydroxy-17-al-ent-abieta-7(8),11(12),13(15)-trien-16,12-olide (3), were isolated from 95% ethanol extract of the roots of Euphorbia wallichii. The structures of the new compounds were elucidated by spectroscopic analysis (NMR, IR, UV, and MS). The isolated diterpenoids were tested in vitro for antimicrobial potentials against 6 pathogenic microorganisms. As a result, compounds 1-3 exhibited some antimicrobial activity against the tested Gram positive bacteria with minimum inhibitory concentration values less than 60 μg/ml.[Formula: see text].
Keywords: Euphorbia wallichii; Euphorbiaceae; antimicrobial activity; ent-abietane-type diterpenoids.