Landscapes of Protein Posttranslational Modifications of African Trypanosoma Parasites

Naiwen Zhang; Ning Jiang; Kai Zhang; Lili Zheng; Di Zhang; Xiaoyu Sang; Ying Feng; Ran Chen; Na Yang; Xinyi Wang; Zhongyi Cheng; Xun Suo; Zhaorong Lun; Qijun Chen

doi:10.1016/j.isci.2020.101074

Landscapes of Protein Posttranslational Modifications of African Trypanosoma Parasites

iScience. 2020 May 22;23(5):101074. doi: 10.1016/j.isci.2020.101074. Epub 2020 Apr 18.

Authors

Naiwen Zhang¹, Ning Jiang¹, Kai Zhang¹, Lili Zheng¹, Di Zhang², Xiaoyu Sang¹, Ying Feng¹, Ran Chen¹, Na Yang², Xinyi Wang³, Zhongyi Cheng⁴, Xun Suo⁵, Zhaorong Lun⁶, Qijun Chen⁷

Affiliations

¹ Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China; The Research Unit for Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang 110866, China.
² Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China.
³ College of Basic Sciences, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China.
⁴ Jingjie PTM Biolab (Hangzhou) Co. Ltd, Hangzhou 310018, China.
⁵ National Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
⁶ State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
⁷ Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China; The Research Unit for Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang 110866, China. Electronic address: qijunchen759@syau.edu.cn.

Abstract

Proteins of all living cells undergo a myriad of post-translational modifications (PTMs) that are critical to multifarious life processes. In this study, we describe the first comprehensive multiple PTM-omics atlas in parallel with quantitative proteome analyses of two representative species of African trypanosomes, Trypanosoma brucei and Trypanosoma evansi. Ten PTM types with approximately 40,000 modified sites and 150 histone marks with a fine map on each protein of the two African trypanosomes were accomplished. The two biologically different trypanosomal species displayed distinct PTM-omic features, regulation pathways, and networks. Modifications in the proteins involved in the redox system were mainly upregulated in T. brucei, whereas proteins associated with motility were predominantly modified in T. evansi. The establishment of a database of multiple PTMs in the two parasites provides us with a deep insight into the biological mechanisms that underpin life processes in trypanosomes with different life cycles.

Keywords: Metabolomics; Proteomics.