A fluoran-based viscosity probe with high-performance for lysosome-targeted fluorescence imaging

Defang Han; Jundan Yi; Chang Liu; Lijuan Liang; Kun Huang; Linhai Jing; Dabin Qin

doi:10.1016/j.saa.2020.118405

A fluoran-based viscosity probe with high-performance for lysosome-targeted fluorescence imaging

Spectrochim Acta A Mol Biomol Spectrosc. 2020 Sep 5:238:118405. doi: 10.1016/j.saa.2020.118405. Epub 2020 May 5.

Authors

Defang Han¹, Jundan Yi¹, Chang Liu², Lijuan Liang³, Kun Huang⁴, Linhai Jing¹, Dabin Qin⁵

Affiliations

¹ Key Laboratory of Chemical Synthesis and Pollution Control of Sichuan Province, School of Chemistry and Chemical Engineering, China West Normal University, Nanchong 637002, China.
² School of Materials Science & Engineering, Tianjin University of Technology, Tianjin 300384, China.
³ Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
⁴ Key Laboratory of Chemical Synthesis and Pollution Control of Sichuan Province, School of Chemistry and Chemical Engineering, China West Normal University, Nanchong 637002, China. Electronic address: hkun2017@cwnu.edu.cn.
⁵ Key Laboratory of Chemical Synthesis and Pollution Control of Sichuan Province, School of Chemistry and Chemical Engineering, China West Normal University, Nanchong 637002, China. Electronic address: qdbkyl@cwnu.edu.cn.

PMID: 32403072
DOI: 10.1016/j.saa.2020.118405

Abstract

A new fluorescent probe Lyso-Fl has been facilely prepared by an esterification reaction of spironolactone fluoran dye Rdi with ethanol, which shows viscosity-selective response by fluorescence. The new probe delivers obvious fluorescence signal enhancement when environmental viscosity changes from 1.01 cP (water) to 1256 cP (98% glycerol). And, both the emission intensity (575 nm) and fluorescence lifetime of Lyso-Fl exhibit individually good linear relationships with the solution viscosity. Besides, Lyso-Fl gives a selective response to viscosity among various biological species and exhibits pH-independent (1-10) fluorescent signals towards viscosity. More importantly, Lyso-Fl shows low cytotoxicity and can be utilized for monitoring of dexamethasone-stimulated viscosity enhancement by cell imaging with excellent lysosome-targeted performance, promoting it a promising fluorescent probe for lysosomal viscosity detection.

Keywords: Cell imaging; Fluorescent probe; Lysosome; Viscosity.

MeSH terms

Biosensing Techniques / methods
Fluoresceins / chemistry*
Fluorescence
Fluorescent Dyes / chemistry*
HeLa Cells
Humans
Lysosomes / chemistry*
Models, Molecular
Optical Imaging / methods
Viscosity

Substances

Fluoresceins
Fluorescent Dyes
fluoran