SNP markers for low molecular glutenin subunits (LMW-GSs) at the Glu-A3 and Glu-B3 loci in bread wheat

PLoS One. 2020 May 12;15(5):e0233056. doi: 10.1371/journal.pone.0233056. eCollection 2020.

Abstract

The content and composition of seed storage proteins is largely responsible for wheat end-use quality. They mainly consist of polymeric glutenins and monomeric gliadins. According to their electrophoretic mobility, gliadins and glutenins are subdivided into several fractions. Glutenins are classified as high molecular weight or low molecular weight glutenin subunits (HMW-GSs and LMW-GSs, respectively). LMW-GSs are encoded by multigene families located at the orthologous Glu-3 loci. We designed a set of 16 single-nucleotide polymorphism (SNP) markers that are able to detect SDS-PAGE alleles at the Glu-A3 and Glu-B3 loci. The SNP markers captured the diversity of alleles in 88 international reference lines and 27 Mexican cultivars, when compared to SDS-PAGE and STS markers, however, showed a slightly larger percent of multiple alleles, mainly for Glu-B3. SNP markers were then used to determine the Glu-1 and Glu-3 allele composition in 54 CIMMYT historical lines and demonstrated to be useful tool for breeding programs to improve wheat end product properties.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Alleles
  • Base Sequence
  • Bread
  • DNA, Plant / genetics
  • Genes, Plant
  • Genetic Markers
  • Glutens / chemistry
  • Glutens / genetics*
  • Molecular Weight
  • Plant Breeding
  • Polymorphism, Single Nucleotide
  • Protein Subunits
  • Sequence Tagged Sites
  • Triticum / genetics*

Substances

  • DNA, Plant
  • Genetic Markers
  • Protein Subunits
  • Glutens
  • glutenin

Grants and funding

Financial support for this study was provided from the CGIAR Research Program on WHEAT and Delivering Genetic Gains in Wheat Project (OPP1133199) managed by Cornell University with financial support from Bill and Melinda Gates Foundation (BMGF).