Data of ureagenesis from ammonia, glutamine and alanine, and mitochondrial aquaporin-8 expression in thioacetamide-treated hepatocytes

Alejo M Capiglioni; María de Luján Alvarez; Raúl A Marinelli

doi:10.1016/j.dib.2020.105632

Data of ureagenesis from ammonia, glutamine and alanine, and mitochondrial aquaporin-8 expression in thioacetamide-treated hepatocytes

Data Brief. 2020 Apr 30:30:105632. doi: 10.1016/j.dib.2020.105632. eCollection 2020 Jun.

Authors

Alejo M Capiglioni¹, María de Luján Alvarez¹, Raúl A Marinelli¹

Affiliation

¹ Instituto de Fisiología Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina. Suipacha 570, 2000 Rosario, Santa Fe, Argentina.

Abstract

We present data about the synthesis of urea from different substrates, i.e., free ammonia, glutamine and alanine in primary cultured rat hepatocytes treated or untreated with the model hepatotoxic agent thioacetamide (TAA). We also provide data about the expression of mitochondrial aquaporin-8 (mtAQP8), a hepatocyte channel protein which facilitates ammonia diffusion into mitochondria to supply the urea cycle. Ammonia-derived ureagenesis was significantly inhibited by about 30% while that from the both amino acids resulted unaffected in TAA-treated hepatocytes. Protein expression of mtAQP8 was decreased by about 80% after TAA treatment. These data can be useful for the understanding of the mechanisms of drug-induced hepatic dysfunction.

Keywords: Alanine; Ammonia; Glutamine; Mitochondrial aquaporin-8; TAA, thioacetamide; Thioacetamide; Ureagenesis; mtAQP8, mitochondrial aquaporin-8.