Quantitative phosphoproteomics analysis reveals that protein modification and sugar metabolism contribute to sprouting in potato after BR treatment

Liqin Li; Mengsheng Deng; Chengcheng Lyu; Jie Zhang; Jie Peng; Chengcheng Cai; Shimin Yang; Liming Lu; Su Ni; Fan Liu; Shunlin Zheng; Liping Yu; Xiyao Wang

doi:10.1016/j.foodchem.2020.126875

Quantitative phosphoproteomics analysis reveals that protein modification and sugar metabolism contribute to sprouting in potato after BR treatment

Food Chem. 2020 Apr 21:325:126875. doi: 10.1016/j.foodchem.2020.126875. Online ahead of print.

Authors

Liqin Li¹, Mengsheng Deng², Chengcheng Lyu³, Jie Zhang⁴, Jie Peng⁵, Chengcheng Cai⁶, Shimin Yang⁷, Liming Lu⁸, Su Ni⁹, Fan Liu¹⁰, Shunlin Zheng¹¹, Liping Yu¹², Xiyao Wang¹³

Affiliations

¹ College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China. Electronic address: liliqin88@163.com.
² College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China. Electronic address: dengmengsheng87@hotmail.com.
³ College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China. Electronic address: chengchengLyu@163.com.
⁴ College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China. Electronic address: ZhangjDMS@outlook.com.
⁵ College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China. Electronic address: gusongke1988@163.com.
⁶ College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China. Electronic address: cccqyj@hotmail.com.
⁷ College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China. Electronic address: yangshimin1@163.com.
⁸ College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China. Electronic address: luliming@sicau.edu.cn.
⁹ College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China. Electronic address: ns13@163.com.
¹⁰ College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China. Electronic address: 541169023@qq.com.
¹¹ College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China. Electronic address: 248977311@qq.com.
¹² College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China. Electronic address: 757336519@qq.com.
¹³ College of Agronomy, Sichuan Agriculture University, Chengdu 611130, China. Electronic address: wxyrtl@163.com.

PMID: 32387993
DOI: 10.1016/j.foodchem.2020.126875

Abstract

Brassinosteroids (BRs), a new class of steroid hormones, are involved in the regulation of plant cell elongation and seed germination. Nevertheless, the molecular mechanism of the effect of BRs on tuber sprouting remains largely unknown. In this study, quantitative phosphoproteomics was employed to investigate the protein phosphorylation changes in sprouting induced by BRs. Our results showed that BRs accelerated the conversion of starch into soluble sugar in tubers. A functional enrichment cluster analysis suggested that the "amino acid metabolism pathway" was upregulated and that "plant hormone signal transduction and protein export" were downregulated. BR treatment also changed the phosphorylation of proteins involved in the BR, ABA, starch and sugar signal transduction pathways, such as serine/threonine-protein kinase (BSK), 14-3-3, alpha-glucan water dikinase (GWD), sucrose-phosphate synthase (SPS), sucrose synthase (SS) and alkaline/neutral invertase (A/N-INV). These results shed more light on the pattern of protein phosphorylation in BR promoting potato sprouting.

Keywords: 1,3-DPG, PubChem CID: 683; 2-DPG, PubChem CID: 59; 3-DPG, PubChem CID: 724; Amylopectin, PubChem CID: 439207; Amylose, PubChem CID: 53477771; Brassinosteroids; Glucose, PubChem CID: 107526; PGAL, PubChem CID: 729; Phosphoproteomics; Potato; Sprouting; Sucrose, PubChem CID: 5988; α-D-Glucose, PubChem CID: 79025; α-D-Glucose-1P, PubChem CID: 65533.