Bioanalytical method validation for the quantification of the chlorogenic acid in Capsicum baccatum through High Performance Liquid Chromatography (HPLC-DAD)

Nathânia S Mendes; Silvia M F Pereira; Mariana B S Arantes; Lorena L Glória; Clara R Nunes; Michel de S Passos; Ivo J C Vieira; Luana P de Moraes; Rosana Rodrigues; Daniela B Oliveira

doi:10.1016/j.foodchem.2020.126929

Bioanalytical method validation for the quantification of the chlorogenic acid in Capsicum baccatum through High Performance Liquid Chromatography (HPLC-DAD)

Food Chem. 2020 Apr 27:325:126929. doi: 10.1016/j.foodchem.2020.126929. Online ahead of print.

Affiliations

¹ Laboratório de Tecnologia de Alimentos, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil.
² Laboratório de Ciências Químicas, Centro de Ciências e Tecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil.
³ Laboratório de Ciências Químicas, Centro de Ciências e Tecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil. Electronic address: curcino@uenf.br.

PMID: 32387988
DOI: 10.1016/j.foodchem.2020.126929

Abstract

The fruit pulp extracts of the species Capsicum baccatum are associated with a number of phenolic compounds. The aim of this study was to validate a bioanalytical method for the quantification of the chlorogenic acid present in the UENF 1613 de C. baccatum accession. The quantification was carried out through HPLC. The results obtained for the extract of C. baccatum indicated selectivity for chlorogenic acid. The values obtained for relative standard deviation, as well as for precision, in terms of repeatability and intermediate precision, did not exceed the maximum of 15% set in the criteria for the acceptance of bioanalytical methods. In terms of recovery, the accuracy rate for the phenolic compound of interest was from 77 to 93%. The validated method through external standardization was applied for the quantification of chlorogenic acid of the UENF accession.

Keywords: Accession; Bioanalytical method; Capsicum baccatum; Chlorogenic acid.