Complementary analyses of the transcriptome and iTRAQ proteome revealed mechanism of ethylene dependent salt response in bread wheat (Triticum aestivum L.)

Qian Ma; Changhai Shi; Chunxue Su; Yiguo Liu

doi:10.1016/j.foodchem.2020.126866

Complementary analyses of the transcriptome and iTRAQ proteome revealed mechanism of ethylene dependent salt response in bread wheat (Triticum aestivum L.)

Food Chem. 2020 Apr 20:325:126866. doi: 10.1016/j.foodchem.2020.126866. Online ahead of print.

Authors

Qian Ma¹, Changhai Shi², Chunxue Su¹, Yiguo Liu³

Affiliations

¹ College of Life Science, Qingdao Agricultural University, Qingdao 266109, China.
² College of Agriculture, Qingdao Agricultural University, Qingdao 266109, China.
³ College of Agriculture, Qingdao Agricultural University, Qingdao 266109, China. Electronic address: yiguoliu@163.com.

PMID: 32387982
DOI: 10.1016/j.foodchem.2020.126866

Abstract

In order to clarify the ethylene dependent salt response mechanism in wheat, 2-week-old wheat seedlings of cultivar 'Qingmai 6' treated with water, sodium chloride (NaCl), NaCl and ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), and NaCl and ethylene signaling inhibitor 1-methylcyclopropene (1-MCP) were collected and analyzed by transcriptional sequencing and isobaric tags for relative and absolute quantitation (iTRAQ) proteomics. At least 1140 proteins and 73,401 genes were identified, and proteins including ribosomal proteins (RPs), nucleoside diphosphate kinases (CDPKs), transaldolases (TALs), beta-glucosidases (BGLUs), phosphoenlpyruvate carboxylases (PEPCs), superoxide dismutases (SODs), and 6-phosphogluconate dehydrogenases (6-PGDHs) were significantly differently expressed. These genes and proteins revealed that ethylene dependent salt response through RPs activation, chaperones synthesis, the reactive oxygen species (ROS) scavenging, and carbohydrate metabolites pathway. Our results provided transcriptomics and proteomics information with respect to the molecular mechanisms of ethylene regualted salt response.

Keywords: 1-MCP (PubChem CID: 151080); ACC (PubChem CID: 535); Bread wheat; Ethylene; Ethylene (PubChem CID: 6325); Proteome; Salt; Sodium chloride (PubChem CID: 5234); Transcriptome; Triticum aestivum.