Phenolic compounds from Mediterranean foods as nutraceutical tools for the prevention of cancer: The effect of honey polyphenols on colorectal cancer stem-like cells from spheroids

Danila Cianciosi; Tamara Y Forbes-Hernández; Johura Ansary; Emilio Gil; Adolfo Amici; Stefano Bompadre; Jesus Simal-Gandara; Francesca Giampieri; Maurizio Battino

doi:10.1016/j.foodchem.2020.126881

Phenolic compounds from Mediterranean foods as nutraceutical tools for the prevention of cancer: The effect of honey polyphenols on colorectal cancer stem-like cells from spheroids

Food Chem. 2020 Apr 25:325:126881. doi: 10.1016/j.foodchem.2020.126881. Online ahead of print.

Authors

Danila Cianciosi¹, Tamara Y Forbes-Hernández², Johura Ansary³, Emilio Gil⁴, Adolfo Amici⁵, Stefano Bompadre⁶, Jesus Simal-Gandara⁷, Francesca Giampieri⁸, Maurizio Battino⁹

Affiliations

¹ Dipartimento di Scienze Cliniche Specialistiche e Odontostomatologiche - Università Politecnica delle Marche, Via Ranieri 65, 60130 Ancona, Italy. Electronic address: d.cianciosi@pm.univpm.it.
² Nutrition and Food Science Group, Department of Analytical and Food Chemistry, CITACA, CACTI, University of Vigo - Vigo Campus, 32004 Ourense, Spain. Electronic address: tforbes@uvigo.es.
³ Dipartimento di Scienze Cliniche Specialistiche e Odontostomatologiche - Università Politecnica delle Marche, Via Ranieri 65, 60130 Ancona, Italy.
⁴ Nutrition and Food Science Group, Department of Biochemistry, Genetics and Immunology, Faculty of Biology, University of Vigo, Vigo, Spain. Electronic address: egil@uvigo.es.
⁵ Dipartimento di Scienze Cliniche Specialistiche e Odontostomatologiche - Università Politecnica delle Marche, Via Ranieri 65, 60130 Ancona, Italy. Electronic address: a.amici@staff.univpm.it.
⁶ Dipartimento Scienze Biomediche e Sanita' Pubblica Università Politecnica delle Marche, Via Ranieri 65, 60130 Ancona, Italy. Electronic address: s.bompadre@univpm.it.
⁷ Nutrition and Bromatology Group, Department of Analytical and Food Chemistry, Faculty of Science, University of Vigo, Ourense Campus, E-32004 Ourense, Spain. Electronic address: jsimal@uvigo.es.
⁸ Nutrition and Food Science Group, Department of Analytical and Food Chemistry, CITACA, CACTI, University of Vigo - Vigo Campus, 32004 Ourense, Spain; Dipartimento di Scienze Cliniche Specialistiche e Odontostomatologiche - Università Politecnica delle Marche, Via Ranieri 65, 60130 Ancona, Italy; College of Food Science and Technology, Northwest University, Xi'an, Shaanxi 710069, China. Electronic address: f.giampieri@univpm.it.
⁹ Nutrition and Food Science Group, Department of Analytical and Food Chemistry, CITACA, CACTI, University of Vigo - Vigo Campus, 32004 Ourense, Spain; International Research Center for Food Nutrition and Safety, Jiangsu University, Zhenjiang 212013, China; Dipartimento di Scienze Cliniche Specialistiche e Odontostomatologiche - Università Politecnica delle Marche, Via Ranieri 65, 60130 Ancona, Italy. Electronic address: m.a.battino@univpm.it.

PMID: 32387951
DOI: 10.1016/j.foodchem.2020.126881

Abstract

Chemoresistance and development of relapses are ascribable to a rare cell population of tumour mass: cancer stem cells (CSCs). Targeting CSCs could increase patients' survival rate and it is important to identify molecules that can act on the main pathways of these cells. Natural bioactive compounds, of which Manuka honey (MH) is rich, could be a good opportunity to target them. This work aims to evaluate the effect of MH on CSCs-like from human colorectal carcinoma (HCT-116 cell line) enriched through the in vitro sphere-forming assay. The results showed that MH reduced the volume of the entire culture of spheroids, affecting also their morphological parameters and induced apoptosis and ROS intracellular accumulation in CSCs-like. In addition, MH decreased the mRNA expression of one of ABC transporters (ABCG2) and affected self-renewal ability through the downregulation of the mRNA expression of one of the receptor membranes of Wnt/β-catenin pathway (Frizzled 7).

Keywords: 3D culture; Cancer stem cells; Chemoresistance; Manuka honey; Self-renewal; Spheroids; Tumorspheres.