Structural peculiarities of lysozyme - PLURONIC complexes at the aqueous-air and liquid-liquid interfaces and in the bulk of aqueous solution

Maria G Chernysheva; Alexey V Shnitko; Alexander L Ksenofontov; Alexander M Arutyunyan; Maxim V Petoukhov; Gennadii A Badun

doi:10.1016/j.ijbiomac.2020.04.221

Structural peculiarities of lysozyme - PLURONIC complexes at the aqueous-air and liquid-liquid interfaces and in the bulk of aqueous solution

Int J Biol Macromol. 2020 May 5:158:721-731. doi: 10.1016/j.ijbiomac.2020.04.221. Online ahead of print.

Authors

Maria G Chernysheva¹, Alexey V Shnitko², Alexander L Ksenofontov³, Alexander M Arutyunyan³, Maxim V Petoukhov⁴, Gennadii A Badun²

Affiliations

¹ Dpt. Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia. Electronic address: chernysheva@radio.chem.msu.ru.
² Dpt. Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia.
³ Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992, Moscow, Russia.
⁴ A. V. Shubnikov Institute of Crystallography of Federal Scientific Research Centre "Crystallography and Photonics" of Russian Academy of Sciences, 119333 Moscow, Russia; A. N. Frumkin Institute of Physical Chemistry and Electrochemistry of Russian Academy of Sciences, 119071 Moscow, Russia.

PMID: 32387357
DOI: 10.1016/j.ijbiomac.2020.04.221

Abstract

Interaction between proteins and synthetic polymers that represent a perspective potential in drug delivery or/and already used in medicine plays a key role in biological functioning of both molecules along with a system as a whole. In present study association between hen egg white lysozyme and Pluronic triblock-copolymers (L121, P123 and F127) in the bulk of the solution as well as at the aqueous-air and liquid-liquid interfaces was analyzed by means of spectroscopic and radiochemical assay. In protein-Pluronic complexes lysozyme keeps the secondary structure (CD and SAXS data results), while fluorescence and UV-analysis indicates changes in the local surrounding of fluorophoric amino acid residues. Radiochemical assay in combination with molecular docking reveals the formation of the complexes, in which proline residues turned to the interface between water and hydrophobic medium.

Keywords: Lysozyme; Lysozyme-Pluronic complex; Pluronic copolymers.