To investigate the anti-proliferative effect of flavonoid nanoparticles on the human ovarian cancer cell line SKOV3. Ten nude mice were intraperitoneally inoculated with the human ovarian cancer SKOV3 cell mixture. The treatment group received tail vein injections with 1.25 mg/kg of flavonol, once every 3 days, 0.2 mL each time, for a total of 12 times. The control group was a tumor model that was injected with 50 mL/L glucose solution; it was observed in Lacquerin Group as the Laccase Nanoparticle Group. Tumor quality was recorded after treatment. The morphology of tumor cells in the two groups was observed by fluorescence microscopy. MTS was used to measure the value of tumor cells in the two groups after administration. Apoptosis of SKOV3 cells was detected by flow cytometry using a tumor cell suspension. The MTT results showed a decreased growth rate of SKOV3 cells with an increase in the mass concentration of flavonoid nanoparticles. The nude mice in the control group had scattered cauliflower-like tumor nodules. The treatment group only showed very small granular tumor nodules. The tumor mass within the treatment group was comparable (P > 0.05), but was lower than that in the control group (P < 0.05). The morphology of the tumor cells in the treatment group became longer and thinner and showed a slender spindle shape. Some high-temperature treated cells even appeared star-shaped, and the cell bodies were significantly broadened. Flow cytometry results showed significantly increased apoptosis of the SKOV3 cells after treatment with flavonoid nanoparticles. The MTS results showed a significantly slowed proliferation rate of SKOV3 cells after two days of administering flavonoid nanoparticles (P < 0.05). Flavonoid nanoparticles were nontoxic, and decreased cell proliferation of and promoted apoptosis in an ovarian cancer cell line.