Effects of Processed Polygonum multiflorum with KIOM Patent on Bone Remodeling-Related Protein Expression in Human Osteoblast-Like SaOS-2 Cells

Dae Uk Kim; Jae Yoon Chung; Seong Chul Jin; Mi Hye Kim; Richard Komakech; Ki-Shuk Shim; Yong-Goo Kim; Woong Mo Yang; Youngmin Kang

doi:10.1155/2020/4168535

Effects of Processed Polygonum multiflorum with KIOM Patent on Bone Remodeling-Related Protein Expression in Human Osteoblast-Like SaOS-2 Cells

Evid Based Complement Alternat Med. 2020 Apr 24:2020:4168535. doi: 10.1155/2020/4168535. eCollection 2020.

Authors

Dae Uk Kim^{1

2}, Jae Yoon Chung³, Seong Chul Jin⁴, Mi Hye Kim⁴, Richard Komakech^{5

6

7}, Ki-Shuk Shim⁸, Yong-Goo Kim⁵, Woong Mo Yang⁴, Youngmin Kang^{5

6}

Affiliations

¹ Oriental Pharmacy, College of Pharmacy, Woosuk University, Wanju-gun, Jeonbuk 55338, Republic of Korea.
² School of Korean Medicine, Pusan National University, Beomeo-ri, Mulgeum-eup, Yangsan, Gyeongsangnam-do 626770, Republic of Korea.
³ College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
⁴ Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
⁵ Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine (KIOM), 111 Geonjae-ro, Naju-si, Jeollanam-do 58245, Republic of Korea.
⁶ University of Science and Technology (UST), Korean Convergence Medicine Major KIOM, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 34054, Republic of Korea.
⁷ Natural Chemotherapeutics Research Institute (NCRI), Ministry of Health, P.O. Box 4864, Kampala, Uganda.
⁸ Herbal Medicine Research Division, Korea Institute of Oriental Medicine (KIOM), 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Republic of Korea.

Abstract

This present study evaluated the effects of processed P. multiflorum on osteogenesis using Sarcoma osteogenic (SaOS-2) cell lines and osteoclastogenesis of bone marrow-derived macrophage cells (BMM) and to elucidate differences in effect on the expression of bone-related proteins between commercially sold P. multiflorum and patented, in vitro-propagated Korea Institute of Oriental Medicine (KIOM) P. multiflorum. Raw P. multiflorum and P. multiflorum that were stir-baked and steamed in black bean juice were compared, and western blotting analysis was performed to investigate the expression of bone remodeling-related proteins in SaOS-2 cells. In the cells treated with P. multiflorum steamed in black bean juice, the expression of RANKL was decreased, whereas that of osteoprotegerin, alkaline phosphatase, Runx2, and osterix was increased. Owing to these results, we conclude that processed P. multiflorum can be used as an alternative treatment for bone diseases such as osteoporosis, osteopenia, periodontitis, and Paget's disease.