Construction of ceRNA Coexpression Network and Screening of Molecular Targets in Colorectal Cancer

Zhao Hui; Wang Zhanwei; Yang Xi; Liu Jin; Zhuang Jing; Han Shuwen

doi:10.1155/2020/2860582

Construction of ceRNA Coexpression Network and Screening of Molecular Targets in Colorectal Cancer

Dis Markers. 2020 Apr 21:2020:2860582. doi: 10.1155/2020/2860582. eCollection 2020.

Authors

Zhao Hui¹, Wang Zhanwei², Yang Xi³, Liu Jin⁴, Zhuang Jing⁵, Han Shuwen³

Affiliations

¹ Department of Gastroenterology, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, 198 Hongqi Road, Huzhou, Zhejiang, China 313000.
² Department of Galactophore, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, 198 Hongqi Road, Huzhou, Zhejiang, China 313000.
³ Department of Oncology, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, No. 198 Hongqi Road, Huzhou, Zhejiang Province, China 313000.
⁴ Department of Pathology, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, No. 198 Hongqi Road, Huzhou, Zhejiang Province, China 313000.
⁵ Graduate School of Nursing, Huzhou University, No. 1 Bachelor Road, Huzhou, Zhejiang Province 313000, China.

Abstract

Objective: To screen some RNAs that correlated with colorectal cancer (CRC).

Methods: Differentially expressed miRNAs, lncRNAs, and mRNAs between cancer tissues and normal tissues in CRC were identified using data from the Gene Expression Omnibus (GEO) database. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and protein-protein interactions (PPIs) were performed to do the functional enrichment analysis. And a lncRNA-miRNA-mRNA network was constructed which correlated with CRC. RNAs in this network were subjected to analyze the relationship with the patient prognosis.

Results: A total of 688, 241, and 103 differentially expressed genes (diff-mRNA), diff-lncRNA, and diff-miRNA were obtained between cancer tissues and normal tissues. A total of 315 edges were obtained in the ceRNA network. lncRNA RP11-108K3.2 and mRNA ONECUT2 correlated with prognosis.

Conclusion: The identified RNAs and constructed ceRNA network could provide great sources for the researches of therapy of the CRC. And the lncRNA RP11-108K3.2 and mRNA ONECUT2 may serve as a novel prognostic predictor of CRC.

MeSH terms

Colorectal Neoplasms / genetics*
Colorectal Neoplasms / metabolism
Gene Expression Regulation, Neoplastic
Gene Regulatory Networks*
Humans
MicroRNAs / genetics*
MicroRNAs / metabolism
Protein Interaction Maps*
RNA, Long Noncoding / genetics*
RNA, Long Noncoding / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism

Substances

MicroRNAs
RNA, Long Noncoding
RNA, Messenger