Streptomyces montanus sp. nov., a novel actinomycete isolated from soil

Int J Syst Evol Microbiol. 2020 May;70(5):3226-3233. doi: 10.1099/ijsem.0.004160.

Abstract

A novel actinomycete, designated strain NEAU-C151T, was isolated from soil collected from Mount Song and characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence indicated that strain NEAU-C151T belongs to the genus Streptomyces and exhibited 97.5, 97.4 and 97.4 % similarities to Streptomyces lincolnensis NRRL 2936T, Streptomyces coacervatus AS-0823T, and Streptomyces longisporus ISP 5166T, respectively. The assignment of strain NEAU-C151T to the genus Streptomyces was confirmed by chemotaxonomic data: anteiso-C15 : 0, C16 : 0, iso-C16 : 0, C16 : 1 (ω7c) and anteiso-C17 : 0 as the major cellular fatty acids; whole-cell sugars contained ribose and glucose; phospholipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), unidentified phospholipid (PL), unidentified lipids (L) and phosphatidylinositol mannoside (PIM); the menaquinones were MK-9(H4), MK-9(H6), MK-10(H2) and MK-9(H8). However, multilocus sequence analysis based on five other house-keeping genes (atpD, gyrB, recA, rpoB, and trpB), DNA-DNA relatedness and phenotypic data showed that strain NEAU-C151T could be distinguished from its closest relatives. Consequently, strain NEAU-C151T represents a novel species of the genus Streptomyces, for which the name Streptomyces montanus sp. nov. is proposed. The type strain is NEAU-C151T (=CGMCC 4.7498T=DSM 107808T).

Keywords: 16S rRNA gene; Streptomyces montanus sp. nov.,; polyphasic taxonomy.

MeSH terms

  • Actinobacteria / genetics
  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Genes, Bacterial
  • Multilocus Sequence Typing
  • Nucleic Acid Hybridization
  • Phospholipids / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Streptomyces / classification*
  • Streptomyces / isolation & purification
  • Vitamin K 2 / analogs & derivatives
  • Vitamin K 2 / chemistry

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Vitamin K 2
  • menaquinone 9