Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation

Hirofumi Kurita; Natsuki Haruta; Yoshito Uchihashi; Takahito Seto; Kazunori Takashima

doi:10.1371/journal.pone.0232724

Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation

PLoS One. 2020 May 6;15(5):e0232724. doi: 10.1371/journal.pone.0232724. eCollection 2020.

Authors

Hirofumi Kurita¹, Natsuki Haruta¹, Yoshito Uchihashi¹, Takahito Seto¹, Kazunori Takashima¹

Affiliation

¹ Department of Applied Chemistry and Life Science, Toyohashi University of Technology, Toyohashi, Japan.

Abstract

DNA damage in the A549 human lung cancer cell line treated with cold plasma irradiation was investigated. We confirmed that cold atmospheric plasma generated reactive oxygen and nitrogen species (RONS) in a liquid, and the intracellular RONS level was increased in plasma-irradiated cells. However, a notable decrease in cell viability was not observed 24 hours after plasma irradiation. Because RONS induce oxidative damage in cells, strand breaks and chemical modification of DNA in the cancer cells were investigated. We found that 8-oxoguanine (8-oxoG) formation as well as DNA strand breaks, which have been thoroughly investigated, were induced by plasma irradiation. In addition, up-regulation of 8-oxoG repair enzyme was observed after plasma irradiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

A549 Cells
Cell Survival / drug effects
DNA / metabolism
DNA Breaks, Double-Stranded / drug effects*
DNA Breaks, Single-Stranded / drug effects*
DNA Glycosylases / metabolism*
DNA Repair*
Guanine / analogs & derivatives*
Guanine / biosynthesis
Humans
Oxidative Stress / drug effects
Plasma Gases / pharmacology*
Reactive Nitrogen Species / metabolism
Reactive Oxygen Species / metabolism
Up-Regulation

Substances

Plasma Gases
Reactive Nitrogen Species
Reactive Oxygen Species
8-hydroxyguanine
Guanine
DNA
DNA Glycosylases
oxoguanine glycosylase 1, human

Grants and funding

This work was partly supported by a Grant-in-Aid for Scientific Research (C) (26390096, 17K05095 to HK) from the Japan Society for the Promotion of Science (JSPS). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. There was no additional external funding received for this study.