Data on the characterization of a platelet lysate and of the system in which it is included (nanoparticles/hydrogel), intended for the treatment of wounds

Sergio Bernal; Sergio Alcalá; Doris Cerecedo; Adriana Ganem-Rondero

doi:10.1016/j.dib.2020.105529

Data on the characterization of a platelet lysate and of the system in which it is included (nanoparticles/hydrogel), intended for the treatment of wounds

Data Brief. 2020 Apr 11:30:105529. doi: 10.1016/j.dib.2020.105529. eCollection 2020 Jun.

Authors

Sergio Bernal¹, Sergio Alcalá², Doris Cerecedo³, Adriana Ganem-Rondero¹

Affiliations

¹ División de Estudios de Posgrado (Tecnología Farmacéutica), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Cuautitlán Izcalli 54740, Estado de México, Mexico.
² Laboratorio de Tecnología Farmacéutica, Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México.
³ Laboratorio de Hematobiología, Escuela Nacional de Medicina y Homeopatía, Sección de Estudios de Posgrado, Instituto Politécnico Nacional, Ciudad de México, México.

Abstract

Platelet lysate has attracted attention for different biomedical applications, including biological processes where cellular proliferation and migration have been altered. Spectroscopic properties of a platelet lysate obtained from human platelets were performed in order to be incorporated in polymeric nanoparticles and then into a Pluronic^Ⓡ F127 hydrogel, intended for wound healing (more details can be found at https://doi.org/10.1016/j.ejps.2020.105231 [1]). The platelet lysate (PL) was assessed by ultraviolet, infrared and circular dichroism spectroscopy. The developed hydrogel was also analyzed by infrared spectroscopy to evaluate if the Pluronic^Ⓡ F127 structure was maintained when the nanoparticles or platelet lysate-loaded nanoparticles were included. The sol-gel transition temperature of the hydrogel was determined through its thermal behavior and by dynamic light scattering.

Keywords: Biuret reaction; Bradford reaction; Characterization; Circular dichroism spectroscopy; Infrared spectroscopy; Platelet lysate; Sol-gel transition temperature; Validation.