Extracellular polymeric substance from Rahnella sp. LRP3 converts available Cu into Cu5(PO4)2(OH)4 in soil through biomineralization process

Hoaithuong Do; Chi Che; Zijun Zhao; Yuqi Wang; Mingtang Li; Xiufang Zhang; Xingmin Zhao

doi:10.1016/j.envpol.2020.114051

Extracellular polymeric substance from Rahnella sp. LRP3 converts available Cu into Cu₅(PO₄)₂(OH)₄ in soil through biomineralization process

Environ Pollut. 2020 May:260:114051. doi: 10.1016/j.envpol.2020.114051. Epub 2020 Jan 23.

Authors

Hoaithuong Do¹, Chi Che¹, Zijun Zhao¹, Yuqi Wang¹, Mingtang Li², Xiufang Zhang¹, Xingmin Zhao¹

Affiliations

¹ College of Resource and Environment, Jilin Agricultural University, Changchun, 130118, China.
² College of Resource and Environment, Jilin Agricultural University, Changchun, 130118, China. Electronic address: limingtang@jlau.edu.cn.

PMID: 32369896
DOI: 10.1016/j.envpol.2020.114051

Abstract

Soil contamination by toxic heavy metals such as copper is a serious problem. In this study, the extracellular polymeric substance (EPS) extracted from Rahnella sp. LRP3 was found with the potential of immobilizing Cu-polluted in soil. The EPS could bond to Cu (II) through functional groups (polysaccharides, amide, proteins, and carboxyl groups), which further developed into the porous sphere with a diameter of 20 μm. Besides, EPS could induce the formation of Cu₅(PO₄)₂(OH)₄ crystal by the biomineralization process. Finally, the EPS in the culture solution reduced 89.4 mg/kg of DTPA-Cu content by 78.99% in soil for 10 d under the condition of 25 °C via biomineralization. The results demonstrated that EPS produced by Rahnella sp. LRP3 will be a promising factor in the remediation of Cu contaminated soil.

Keywords: Biomineralization; Copper; Extracellular polymeric substance (EPS); Rahnella sp. LRP3; Remediation.

MeSH terms

Biomineralization*
Copper / chemistry*
Extracellular Polymeric Substance Matrix*
Rahnella / drug effects
Rahnella / physiology*
Soil / chemistry*

Substances

Soil
Copper