Caught in the act: structural dynamics of replication origin activation and fork progression

Biochem Soc Trans. 2020 Jun 30;48(3):1057-1066. doi: 10.1042/BST20190998.

Abstract

This review discusses recent advances in single-particle cryo-EM and single-molecule approaches used to visualise eukaryotic DNA replication reactions reconstituted in vitro. We comment on the new challenges facing structural biologists, as they turn to describing the dynamic cascade of events that lead to replication origin activation and fork progression.

Keywords: in silico reconstitution; DNA replication; biochemistry; cryo-electron microscopy; single molecule.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Adenosine Triphosphatases / metabolism
  • Cell Cycle
  • Computer Simulation
  • Cryoelectron Microscopy / methods*
  • DNA / chemistry
  • DNA / genetics*
  • DNA Replication*
  • Eukaryota
  • Hydrolysis
  • In Vitro Techniques
  • Protein Conformation
  • Replication Origin*
  • Single Molecule Imaging

Substances

  • DNA
  • Adenosine Triphosphatases