Development of an electrochemical biosensor for phylogenetic analysis of Amaryllidaceae based on the enhanced electrochemical fingerprint recorded from plant tissue

Li Fu; Yuhong Zheng; Pengcong Zhang; Haoyang Zhang; Yuting Xu; Jingtao Zhou; Huaiwei Zhang; Hassan Karimi-Maleh; Guosong Lai; Shichao Zhao; Weitao Su; Jinhong Yu; Cheng-Te Lin

doi:10.1016/j.bios.2020.112212

Development of an electrochemical biosensor for phylogenetic analysis of Amaryllidaceae based on the enhanced electrochemical fingerprint recorded from plant tissue

Biosens Bioelectron. 2020 Jul 1:159:112212. doi: 10.1016/j.bios.2020.112212. Epub 2020 Apr 15.

Authors

Li Fu¹, Yuhong Zheng², Pengcong Zhang³, Haoyang Zhang⁴, Yuting Xu⁴, Jingtao Zhou⁴, Huaiwei Zhang⁴, Hassan Karimi-Maleh⁵, Guosong Lai⁶, Shichao Zhao⁴, Weitao Su⁴, Jinhong Yu⁷, Cheng-Te Lin⁸

Affiliations

¹ College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, 310018, PR China. Electronic address: fuli@hdu.edu.cn.
² Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, PR China. Electronic address: zhengyuhong@cnbg.net.
³ Hangzhou Botanical Garden, Hangzhou, Zhejiang, 310013, PR China.
⁴ College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, 310018, PR China.
⁵ School of Resources and Environment, University of Electronic Science and Technology of China, P.O. Box 611731, Xiyuan Ave, Chengdu, PR China.
⁶ Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, Huangshi, 435002, PR China.
⁷ Key Laboratory of Marine New Materials and Related Technology, Zhejiang Key Laboratory of Marine Materials and Protection Technology, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China.
⁸ Key Laboratory of Marine New Materials and Related Technology, Zhejiang Key Laboratory of Marine Materials and Protection Technology, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China. Electronic address: linzhengde@nimte.ac.cn.

PMID: 32364933
DOI: 10.1016/j.bios.2020.112212

Abstract

A biosensor has been developed based on disposable screen-printed electrode for recording the electrochemical fingerprint of plant leaf tissue. A thin layer of polydopamine functionalized graphene sheets was coated on the plant tissue modified electrode for signal enhancement. The voltammetric data recorded under different buffer solutions can be derived as patterns for species identification. As the distribution of electrochemical active compounds in plants is controlled by genes, these fingerprints can reflect differences at the genetic level between species. Therefore, the electrochemical fingerprint of plant tissues can be used for phylogenetic research without qualitative analysis. 19 species of Amaryllidaceae including A. africanus, Clivia miniata, Clivia nobilis, Crinum firmifolium, Crinum latifolium, Crinum moorei, Curculiga gracilis, Cyrtanthus breviflorus, Habranthus robustus, Haemanthus albiflos, Haemathus multiflorus, Hippeastrum rutilum, Hymenocallis littoralis, Leucojum aestivum, Sprekelia formosissima, Tulbaghia violacea, Zephyranthes grandiflora, Zephyranthes macrosiphon and Zephyranthes minima have been selected deliberately. The dendrogram deduced from the electrochemical fingerprint was compared with the molecular phylogenetics. The results indicate the electrochemical fingerprint-based phylogenetic study is a persuasive methodology for plant phylogenetic analysis.

Keywords: Amaryllidaceae; Electrochemical fingerprints; Plant identification; Solid state electrochemistry; Taxonomic sensing.

MeSH terms

Amaryllidaceae / chemistry*
Biosensing Techniques*
Electrochemical Techniques*
Phylogeny*
Plants / classification*
Plants / genetics*