Evaluation of MALDI-TOF MS as a tool for detection of Listeria spp. directly from selective enrichment broth from food and stool samples

J Microbiol Methods. 2020 Jun:173:105936. doi: 10.1016/j.mimet.2020.105936. Epub 2020 Apr 30.

Abstract

We evaluated the detection of Listeria spp. using MALDI-TOF MS directly in enrichment broths, without isolated colonies, with naturally contaminated food and stool samples. The success rate was 77%. Considering the reduced time for diagnosis and the success rate, this is a promising screening tool, but more tests are needed to determine its viability.

Keywords: Enrichment broth; Foodborne pathogen detection; Listeria monocytogenes; Listeria spp.; MALDI-TOF MS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Bacteriological Techniques / methods*
  • Brazil
  • Culture Media
  • Feces / microbiology*
  • Food
  • Food Microbiology / methods*
  • Foodborne Diseases / diagnosis
  • Foodborne Diseases / microbiology
  • Humans
  • Listeria / isolation & purification*
  • Listeria monocytogenes / isolation & purification
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Culture Media