Sulfated galactan from Halymenia dilatata enhance the antioxidant properties and prevents Aeromonas hydrophila infection in tilapia fish: In vitro and in vivo study

Manoharan Vinosha; Subramanian Palanisamy; Ravichandran Anjali; Changsheng Li; Khamphone Yelithao; Thangapandi Marudhupandi; Mehdi Tabarsa; SangGuan You; Narayanasamy Marimuthu Prabhu

doi:10.1016/j.ijbiomac.2020.04.212

Sulfated galactan from Halymenia dilatata enhance the antioxidant properties and prevents Aeromonas hydrophila infection in tilapia fish: In vitro and in vivo study

Int J Biol Macromol. 2020 Apr 28:158:569-579. doi: 10.1016/j.ijbiomac.2020.04.212. Online ahead of print.

Authors

Manoharan Vinosha¹, Subramanian Palanisamy², Ravichandran Anjali¹, Changsheng Li³, Khamphone Yelithao⁴, Thangapandi Marudhupandi⁵, Mehdi Tabarsa⁶, SangGuan You², Narayanasamy Marimuthu Prabhu⁷

Affiliations

¹ Disease Control and Prevention Lab, Department of Animal Health and Management, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India.
² East Coast Life Sciences Institute, Gangneung-Wonju National University, 120 Gangneung, Gangwon 210-720, Republic of Korea; Department of Marine Food Science and Technology, Gangneung-Wonju National University, 120 Gangneungdaehangno, Gangneung, Gangwon 210-702, Republic of Korea.
³ Department of Marine Food Science and Technology, Gangneung-Wonju National University, 120 Gangneungdaehangno, Gangneung, Gangwon 210-702, Republic of Korea.
⁴ Department of Marine Food Science and Technology, Gangneung-Wonju National University, 120 Gangneungdaehangno, Gangneung, Gangwon 210-702, Republic of Korea; Deparment of Food Science and Technology, Souphanouvong University, 13th North rout Laungprabang, Lao People's Democratic Republic.
⁵ Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Science Campus, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India.
⁶ Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, P.O. Box 46414-356, Noor, Iran.
⁷ Disease Control and Prevention Lab, Department of Animal Health and Management, Alagappa University, Karaikudi, 630 003, Tamil Nadu, India. Electronic address: 71gnmprabhu@gmail.com.

PMID: 32360202
DOI: 10.1016/j.ijbiomac.2020.04.212

Abstract

The structural characterization and pharmaceutical perspective of sulfated galactan from Halymenia dilatata (Hd-SG) were reported in this study. The Hd-SG consists of carbohydrate (58.5 ± 0.9%), sulfate (28.7 ± 0.9%) and protein (2.7 ± 0.2%). The existence of carbon (28.14%), hydrogen (5.50%), nitrogen (0.51%) and sulfur (8.26%) was confirmed in CHNS analysis. The Hd-SG was mainly comprising of galactose and mannose connected by (1 → 4)-glycosidic linkages, and it shows the molecular weight of 900.9 × 10³ g/mol in high-performance size exclusion chromatography (HPSEC). The Hd-SG exhibited the dose depended on antioxidant activities. The in vitro and in vivo studies proved the antibacterial efficacy of Hd-SG against Aeromonas hydrophila. The pre-treated Oreochromis fish with Hd-SG (2.0 g/0.1 kg of feed) showed the highest survival, antioxidant, and improved histological changes than the fish infected with A. hydrophila alone. These results concluded that the isolated Hd-SG has extensive therapeutic properties, and it can be used as preventive medicine.

Keywords: Aeromonas hydrophila; Halymenia dilatata; Sulfated galactans.