A strip test for the optical determination of influenza virus H3 subtype using gold nanoparticle coated polystyrene latex microspheres

Mikrochim Acta. 2020 Apr 30;187(5):306. doi: 10.1007/s00604-020-04255-1.

Abstract

A strip test is described for the optical determination of influenza virus H3 subtype. It utilizes gold nanoparticle (AuNP) coated polystyrene latex microspheres (PS) as the label and a sandwich format. The AuNP and PS particles were linked using monoclonal antibodies against influenza virus as the bridge. Under the optimal conditions, the visual detection limit of the AuNP-PS-based strip test was as low as 1/16 hemagglutination unit (HAU). It was 64 times higher than that of 10 nm (4 HAU) AuNP-based strip tests. Quantitative analysis showed that the detection limit of the AuNP-PS-based strip is 0.016 HAU. The AuNP-PS-based strip test showed no cross-reactivity to the other subtypes (H1, H5, H7, or H9) of influenza viruses. Graphical abstract .

Keywords: Influenza virus; Lateral flow immunoassay; Nanocomposite; Point-of-care test; Visual determination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Immobilized / immunology
  • Antibodies, Monoclonal / immunology
  • Gold / chemistry
  • Immunoassay / instrumentation
  • Immunoassay / methods*
  • Influenza A virus / immunology
  • Influenza A virus / isolation & purification*
  • Limit of Detection
  • Metal Nanoparticles / chemistry*
  • Microspheres*
  • Polystyrenes / chemistry*

Substances

  • Antibodies, Immobilized
  • Antibodies, Monoclonal
  • Polystyrenes
  • Gold