Integrated Human Immunodeficiency Virus Type 1 Sequence in J-Lat 10.6

Cheng-Han Chung; Anthony R Mele; Alexander G Allen; Robert Costello; Will Dampier; Michael R Nonnemacher; Brian Wigdahl

doi:10.1128/MRA.00179-20

Integrated Human Immunodeficiency Virus Type 1 Sequence in J-Lat 10.6

Microbiol Resour Announc. 2020 Apr 30;9(18):e00179-20. doi: 10.1128/MRA.00179-20.

Authors

Cheng-Han Chung^#^{1

2}, Anthony R Mele^#^{1

2}, Alexander G Allen^{1

2}, Robert Costello^{1

2}, Will Dampier^{1

2}, Michael R Nonnemacher^{3

2

4}, Brian Wigdahl^{3

2

4

5}

Affiliations

¹ Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA.
² Center for Molecular Virology and Translational Neuroscience, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA.
³ Department of Microbiology and Immunology, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA mrn25@drexel.edu bw45@drexel.edu.
⁴ Center for Clinical and Translational Medicine, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA.
⁵ Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, USA.

^# Contributed equally.

Abstract

The full length of HIV/R7/E^-/GFP integrated in the J-Lat 10.6 cell line was sequenced in this study. The single copy of the integrated virus, including the breakpoints from the human chromosome to the provirus, was amplified by two separate PCRs. A 10,200-bp genome sequence was acquired, analyzed, and deposited in GenBank.

Abstract

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