Purpose: To study the effect of Lactobacillus E6-1 on proliferation and apoptosis of human oral cancer cell line Cal-27 in vitro.
Methods: MTT assay was carried out by different concentrations of Lactobacillus E6-1 on Cal-27 to detect the inhibitory effect on cell proliferation, and the effect on DNA was detected by agarose gel electrophoresis. The expression levels of cyt-c, caspase-9 and caspase-3 in each group was detected by Western blot. The data were analyzed with SPSS 21.0 software package.
Results: The results of MTT assay showed that E6-1 had obvious inhibitory effect on Cal-27 in a dose-dependent manner. DNA ladder in Cal-27 cells was induced by different concentrations of E6-1 (10, 20, 40 mg/mL). Compared with blank control group, Western blot results showed that the expression of cyt-c, caspase-9 and caspase-3 increased significantly(P<0.05).
Conclusions: Lactobacillus E6-1 can inhibit the proliferation of Cal-27 and effectively induce apoptosis of tumor cells In vitro.