Expression of lamin C2 in mammalian oocytes

PLoS One. 2020 Apr 28;15(4):e0229781. doi: 10.1371/journal.pone.0229781. eCollection 2020.

Abstract

Lamin C2 (LMN C2) is a short product of the lamin a gene. It is a germ cell-specific lamin and has been extensively studied in male germ cells. In this study, we focussed on the expression and localization of LMN C2 in fully-grown germinal vesicle (GV) oocytes. We detected LMN C2 in the fully-grown germinal vesicle oocytes of various mammalian species with confirmation done by immunoblotting the wild type and Lmnc2 gene deleted testes. Expression of LMN C2 tagged with GFP showed localization of LMN C2 to the nuclear membrane of the oocyte. Moreover, the LMN C2 protein notably disappeared after nuclear envelope breakdown (NEBD) and the expression of LMN C2 was significantly reduced in the oocytes from aged females and ceased altogether during meiotic maturation. These results provide new insights regarding LMN C2 expression in the oocytes of various mammalian species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Female
  • Gene Expression Regulation, Developmental / genetics
  • Germ Cells / growth & development
  • Laminin / genetics*
  • Male
  • Meiosis / genetics
  • Mice
  • Mice, Knockout
  • Nuclear Envelope / genetics
  • Oocytes / growth & development*
  • Oocytes / metabolism
  • Oogenesis / genetics*
  • Ovary / growth & development*
  • RNA, Messenger / genetics
  • Spermatocytes / growth & development
  • Testis / growth & development

Substances

  • Laminin
  • RNA, Messenger
  • lamin C2

Grants and funding

This research was funded by GACR18-19395S and EXCELLENCECZ.02.1.01/0.0/0.0/15_003/0000460 OP RDE and Institutional Research Concept RVO67985904. MA received support from the German Research Foundation (DFG), grant Al 1090/2-1. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.