Quantitative analysis of interactive behavior of mitochondria and lysosomes using structured illumination microscopy

Qixin Chen; Xintian Shao; Mingang Hao; Hongbao Fang; Ruilin Guan; Zhiqi Tian; Miaoling Li; Chenran Wang; Liangnian Ji; Hui Chao; Jun-Lin Guan; Jiajie Diao

doi:10.1016/j.biomaterials.2020.120059

Quantitative analysis of interactive behavior of mitochondria and lysosomes using structured illumination microscopy

Biomaterials. 2020 Aug:250:120059. doi: 10.1016/j.biomaterials.2020.120059. Epub 2020 Apr 19.

Authors

Qixin Chen¹, Xintian Shao², Mingang Hao³, Hongbao Fang³, Ruilin Guan⁴, Zhiqi Tian³, Miaoling Li⁵, Chenran Wang³, Liangnian Ji⁴, Hui Chao⁶, Jun-Lin Guan⁷, Jiajie Diao⁸

Affiliations

¹ Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267, USA; Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, Sichuan, 646000, China.
² Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267, USA; Shandong Academy of Pharmaceutical Science, Key Laboratory of Biopharmaceuticals, Engineering Laboratory of Polysaccharide Drugs, National-Local Joint Engineering Laboratory of Polysaccharide Drugs, Jinan, 250101, China.
³ Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267, USA.
⁴ MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China.
⁵ Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, Sichuan, 646000, China.
⁶ MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou, 510275, China. Electronic address: ceschh@mail.sysu.edu.cn.
⁷ Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267, USA. Electronic address: guanjl@ucmail.uc.edu.
⁸ Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267, USA. Electronic address: jiajie.diao@uc.edu.

Abstract

Super-resolution optical microscopy has extended the spatial resolution of cell biology from the cellular level to the nanoscale, enabling the observation of the interactive behavior of single mitochondria and lysosomes. Quantitative parametrization of interactions between mitochondria and lysosomes under super-resolution optical microscopy, however, is currently unavailable, which has severely limited our understanding of the molecular machinery underlying mitochondrial functionality. Here, we introduce an M-value to quantitatively investigate mitochondria and lysosome contact (MLC) and mitophagy under structured illumination microscopy. We found that the M-value for an MLC is typically less than 0.4, whereas in mitophagy it ranges from 0.5 to 1.0. This system permits further investigation of the detailed molecular mechanism governing the interactive behavior of mitochondria and lysosomes.

Keywords: Lysosome; Membrane fusion; Mitochondria; Mitophagy; Super-resolution imaging.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Lighting*
Lysosomes / metabolism
Microscopy*
Mitochondria
Mitophagy

Abstract

Publication types

MeSH terms

Grants and funding