In situ detection of Australian gill-associated virus with a yellow head virus gene probe

Kathy F-J Tang; Kirsten M Spann; Leigh Owens; Donald V Lightner

doi:10.1016/S0044-8486(01)00666-4

In situ detection of Australian gill-associated virus with a yellow head virus gene probe

Aquaculture. 2002 Feb 28;205(1):1-5. doi: 10.1016/S0044-8486(01)00666-4. Epub 2002 Jan 29.

Authors

Kathy F-J Tang¹, Kirsten M Spann², Leigh Owens³, Donald V Lightner¹

Affiliations

¹ Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721, USA.
² Co-operative Research Centre for Aquaculture, CSIRO Livestock Industries, 120 Meiers Road, Indooroopilly 4068, Australia.
³ Department of Biomedical and Tropical Veterinary Sciences, James Cook University of North Queensland, Townsville, Queensland 4811, Australia.

Abstract

A digoxigenin-labeled gene probe for yellow head virus (YHV) was used to detect gill-associated virus (GAV) in Penaeus monodon from Australia via in situ hybridization. In GAV-infected shrimp, positively reacting tissues included: lymphoid organ, gills, antennal gland, and cuticular epithelium of the stomach. This demonstrates that a YHV probe can be used as a diagnostic tool for GAV and supports previous suggestions that these two viruses are closely related.

Keywords: Gill-associated virus; In situ hybridization; Yellow head virus.