Cloning and characterisation of type I interferon receptor 1 in orange-spotted grouper (Epinephelus coioides) for response to nodavirus infection

Zhi Zhuang Tang; Ting-Yu Wang; Young-Mao Chen; Tzong-Yueh Chen

doi:10.1016/j.fsi.2020.04.044

Cloning and characterisation of type I interferon receptor 1 in orange-spotted grouper (Epinephelus coioides) for response to nodavirus infection

Fish Shellfish Immunol. 2020 Jun:101:302-311. doi: 10.1016/j.fsi.2020.04.044. Epub 2020 Apr 23.

Authors

Zhi Zhuang Tang¹, Ting-Yu Wang², Young-Mao Chen³, Tzong-Yueh Chen⁴

Affiliations

¹ Laboratory of Molecular Genetics, Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan; Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan; Translational Center for Marine Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan; Agriculture Biotechnology Research Center, National Cheng Kung University, Tainan, 70101, Taiwan.
² Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan; Translational Center for Marine Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan; Agriculture Biotechnology Research Center, National Cheng Kung University, Tainan, 70101, Taiwan.
³ Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan; Translational Center for Marine Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan; Agriculture Biotechnology Research Center, National Cheng Kung University, Tainan, 70101, Taiwan; University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan; Bachelor Degree Program in Marine Biotechnology, College of Life Sciences, National Taiwan Ocean University, Keelung, 20224, Taiwan; Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, 20224, Taiwan.
⁴ Laboratory of Molecular Genetics, Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan; Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan; Translational Center for Marine Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan; Agriculture Biotechnology Research Center, National Cheng Kung University, Tainan, 70101, Taiwan; University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, 70101, Taiwan. Electronic address: ibcty@mail.ncku.edu.tw.

PMID: 32335315
DOI: 10.1016/j.fsi.2020.04.044

Abstract

Grouper is known as a highly economical teleost species in the Asian aquaculture industry; however, intensive culture activities easily cause disease outbreak, especially viral disease. For the prevention of viral outbreaks, interferon (IFN) is among the major defence systems being studied in different species. Fish type I IFNs are known to possess antiviral properties similar to mammalian type I IFNs. In order to stimulate antiviral function, IFN will bind to its cognate receptor, the type I interferon receptor (IFNAR), composed of heterodimeric receptor subunits known as IFNAR1 and IFNΑR2. The binding of type I interferon to receptors assists in the transduction of signals from the external to internal environments of cells to activate biological responses. In order to study the function of IFN, we first need to understand IFN receptors. In this study, we cloned and identified IFNAR1 in orange-spotted grouper (osgIFNAR1) and noted the up-regulated mRNA expression of the receptor and downstream effectors in the head kidney cells with cytokine treatment. The transcriptional expression of osgIFNAR1, which is characterised using polyinosinic-polycytidylic acid (poly[I:C]) and lipopolysaccharide (LPS) treatments, indicated the involvement of osgIFNAR1 in the immune response of grouper. The subcellular localisation of osgIFNAR1 demonstrated scattering across the grouper cell. Viral infection showed the negative feedback regulation of osgIFNAR1 in grouper larvae. Further loss of function of IFNAR1 showed a decreased expression of the virus. This study reported the identification of osgIFNAR1 and characterisation of receptor sensitivity towards immunostimulants, cytokine response, and viral challenge in the interferon pathway of orange-spotted grouper and possible different role of the receptor in viral production. Together, these results provide a frontline report of the potential function of osgIFNAR1 in the innate immunity of teleost.

Keywords: Nervous necrosis virus; Orange-spotted grouper; Type I interferon Receptor.

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Bass / genetics*
Bass / immunology*
Cytokines / metabolism
Fish Diseases / immunology*
Fish Diseases / virology
Fish Proteins / chemistry
Fish Proteins / genetics
Fish Proteins / immunology
Gene Expression Profiling / veterinary
Gene Expression Regulation / immunology*
Immunity, Innate / genetics*
Lipopolysaccharides / administration & dosage
Nodaviridae / physiology
Phylogeny
Poly I-C / administration & dosage
RNA Virus Infections / immunology
RNA Virus Infections / veterinary
RNA Virus Infections / virology
Receptor, Interferon alpha-beta / chemistry
Receptor, Interferon alpha-beta / genetics*
Receptor, Interferon alpha-beta / immunology*
Sequence Alignment / veterinary

Substances

Cytokines
Fish Proteins
Lipopolysaccharides
Receptor, Interferon alpha-beta
Poly I-C