Method to decellularize the human chorion membrane

Laura P Frazão; Joana Vieira-de-Castro; Cristina Nogueira-Silva; Nuno M Neves

doi:10.1016/bs.mcb.2019.11.002

Method to decellularize the human chorion membrane

Methods Cell Biol. 2020:157:23-35. doi: 10.1016/bs.mcb.2019.11.002. Epub 2019 Nov 27.

Authors

Laura P Frazão¹, Joana Vieira-de-Castro¹, Cristina Nogueira-Silva², Nuno M Neves³

Affiliations

¹ I3B's-Research Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho: 3Bs Research Group, Guimarães, Portugal,; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal.
² ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal; Life and Health Sciences Research Institute, School of Medicine, University of Minho, Braga, Portugal; Department of Obstetrics and Gynecology, Hospital de Braga, Braga, Portugal.
³ I3B's-Research Institute on Biomaterials, Biodegradables and Biomimetics of University of Minho: 3Bs Research Group, Guimarães, Portugal,; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal. Electronic address: nuno@i3bs.uminho.pt.

PMID: 32334717
DOI: 10.1016/bs.mcb.2019.11.002

Abstract

The human placenta is considered a biological waste, thus it is a great source of extracellular matrix (ECM) proteins. The human chorion membrane (HCM) is a membrane that composes the human placenta and is constituted by collagens type I, II, IV, V and VI, fibronectin and laminin. To the best of our knowledge, the potential of HCM alone is largely unexplored as a substrate to be used in tissue engineering and regenerative medicine. In this work, we describe, for the first time, the process and method to decellularize the chorion membrane alone. To verify the success of the decellularization protocol, the presence and distribution of cell nuclei and double-stranded DNA were quantified and analyzed by DAPI staining, PicoGreen and electrophoresis. After the decellularization protocol an ECM compact and handleably membrane is obtained, the decellularized human chorion membrane (dHCM).

Keywords: Chorion membrane; Decellularization; Extracellular matrix; Membranes; Placenta.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Nucleus / chemistry
Chorion / cytology*
DNA / analysis
Extracellular Matrix Proteins / analysis
Extracellular Matrix*
Female
Humans
Placenta / cytology
Pregnancy
Regenerative Medicine / methods
Tissue Engineering / methods*
Tissue Scaffolds*

Substances

Extracellular Matrix Proteins
DNA