Rapid on-site detection of genetically modified soybean products by real-time loop-mediated isothermal amplification coupled with a designed portable amplifier

Hui Wu; Xianjun Zhang; Baoqiang Wu; Cheng Qian; Fang Zhang; Liu Wang; Zunzhong Ye; Jian Wu

doi:10.1016/j.foodchem.2020.126819

Rapid on-site detection of genetically modified soybean products by real-time loop-mediated isothermal amplification coupled with a designed portable amplifier

Food Chem. 2020 Apr 15:323:126819. doi: 10.1016/j.foodchem.2020.126819. Online ahead of print.

Authors

Hui Wu¹, Xianjun Zhang², Baoqiang Wu², Cheng Qian¹, Fang Zhang³, Liu Wang⁴, Zunzhong Ye⁵, Jian Wu¹

Affiliations

¹ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
² Hangzhou An Yu Technologies Co.,Ltd., Hangzhou 310021, China.
³ College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China.
⁴ Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, State Key Laboratory for Quality and Safety of Agro-products, Hangzhou 310021, China. Electronic address: wangliually@126.com.
⁵ College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: zzye@zju.edu.cn.

PMID: 32334306
DOI: 10.1016/j.foodchem.2020.126819

Abstract

Herein, we developed a rapid method for detection of genetically modified soybean (GTS 40-3-2) products using loop-mediated isothermal amplification (LAMP). A crude 5-minute extraction method was established for DNA extraction from soybeans and soybean products. LAMP reaction for CaMV35S promoter was optimized and the fastest threshold time (T_t) was 14 min with 4 mM magnesium ions at 63 °C. A portable instrument was designed to perform real-time LAMP in the field. As little as 0.1% GM soybean, specifically, or 0.5% GM ingredients in Chinese traditional tofu could be detected in 30 min from sampling to results. We used this method to further assess other five soybean products to determine whether they contained transgenic ingredients and compared the results with those obtained using PCR, which suggested the proposed method was applicable for rapid detection of genetically modified soybean in food products.

Keywords: Genetically modified; Loop-mediated isothermal amplification; On-site detection; Portable instrument; Soybean products.