Abstract: Maillard reaction and Maillard reaction products (MRPs) are commonly used in food industry, with various sensory and functional properties. Bovine bone marrow extract (BBME) was subjected to enzymatic hydrolysis, followed by the Maillard reaction to enhance its sensory attributes. The antioxidant and antihypertensive activities of BBME, its hydrolysate and their MRPs were assessed. (37) orthogonal experimental design was used to obtain the optimal reaction conditions for generation of MRPs with improved sensory characteristics. The optimal conditions were: processing temperature, 120 °C; processing time, 60 min; xylose concentration, 2.5% (w/v); cysteine concentration, 2%; Vitamin B1, 1.2%; Hydrolyzed vegetable protein, 6%; and sample to liquid ratio, 1:1. The total content of free amino acid in BBME decreased from 86.18 to 25.50 g L-1 in EH-BBME-MRPs. In addition, EH-BBME and EH-BBME-MRPs contained significantly higher amount of low molecular weight peptides (< 1000 Da; 47.2% and 21.84%, respectively) compared to other samples. They also exhibited enhanced antioxidant activities against DPPH, ABTS and hydroxyl free radicals, and presented improved FRAP activity and reducing power. EH-BBME-MRPs also exhibited higher antihypertensive activity compared to other samples. The results indicate that MRPs derived from BBME hydrolysate are promising components for improving food flavor and also provide health benefits.
Keywords: Antioxidant activity; Bovine bone marrow extract; Free radical scavenging activity; Hydrolysate; Maillard reaction.
© Association of Food Scientists & Technologists (India) 2019.