Abstract
Here, we describe a procedure to fluorescently contrast the nuclear boundary using the lipophilic carbocyanine dye DiI in cultured human cells. Our procedure is simple and is applicable to detect nuclear boundary defects, which may be relevant to studies on nuclear envelope dynamics, micronuclei formation and cancer biology.
Abbreviations:
DiI: 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate; DiO: 3,3'-dioctadecyloxacarbocyanine perchlorate; NE: nuclear envelope; RanBP2: Ran-binding protein 2/Nucleoporin 358.
Keywords:
Cancer; lipid membrane; lipophilic carbocyanine dye; micronucleus; nuclear envelope.
MeSH terms
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Animals
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Biomarkers / metabolism
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Cell Line
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Cell Line, Tumor
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Fluorescent Dyes / analysis*
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Fluorescent Dyes / chemistry
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Gene Expression
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HeLa Cells
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Hepatocytes / metabolism
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Hepatocytes / ultrastructure
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Humans
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Methylamines / analysis*
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Methylamines / chemistry
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Mice
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Molecular Chaperones / genetics
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Molecular Chaperones / metabolism
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Muscle Cells / metabolism
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Muscle Cells / ultrastructure
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Nuclear Envelope / metabolism
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Nuclear Envelope / ultrastructure*
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Nuclear Pore Complex Proteins / genetics
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Nuclear Pore Complex Proteins / metabolism
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Optical Imaging / methods*
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Staining and Labeling / methods*
Substances
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1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarboxyamine perchlorate
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Biomarkers
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Fluorescent Dyes
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Methylamines
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Molecular Chaperones
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Nuclear Pore Complex Proteins
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ran-binding protein 2