Cd heavy metal and plants, rather than soil nutrient conditions, affect soil arbuscular mycorrhizal fungal diversity in green spaces during urbanization

Abstract

Urbanization accelerates pollution and habitat fragmentation, and the mechanism that shapes the arbuscular mycorrhizal (AM) fungal community in urban ecosystem still remains poorly understood. In this study, soil samples from 23 sites (from rural to urban), belonging to 4 green space types (country park, Co; urban park, Pa; roadside green space1, RoP1; and roadside green space2, RoP2), were collected to assess the effects of the urbanization on the AM fungal diversity. Using 454 pyrosequencing, a total of 79 AM fungal OTUs were uncovered. We found that urbanization showed a neutral effect on Shannon diversity, Simpson diversity, Pielou diversity, and community composition of the AM fungi. Within urban areas, the composition of AM fungal community was significantly different between RoP1 and RoP2. The db-RDA analysis of RoP1 and RoP2 revealed that the soil Cd accounted for the largest community composition variation, with an explanation rate of 20.5%, followed by the SOC (15.1%). Across 23 sites, Cd may have an obvious ecological toxicity on AM fungi, with significantly negative correlations between the soil Cd content and the AM fungal species richness and evenness. The AM fungal community also indicated significantly Mantel correlation with the soil Cd contents. Additionally, high herbaceous richness promoted rich AM fungi. The herbaceous composition, not the richness, has a significant impact on the AM fungal community composition. This study suggests that the toxicity of Cd from traffic should receive more attention during urban green space construction and management, and reasonable plant configuration contributed to the maintenance of the AM fungal community.

Keywords: Arbuscular mycorrhizal fungi; Cd; Diversity; Herbaceous plants; Urban green space.