Exogenously supplied silicon (Si) improves cadmium tolerance in pepper (Capsicum annuum L.) by up-regulating the synthesis of nitric oxide and hydrogen sulfide

Abstract

The current research was aimed to observe the interactive role of silicon-generated hydrogen sulfide (H₂S) and nitric oxide (NO) on tolerance of pepper (Capsicum annum L.) plants to cadmium (Cd). Thus, the pepper plants were subjected to control (no Cd) or cadmium stress with and without Si supplementation. Significant decreases were found in plant dry weights, water potential, PSII maximum efficiency, glutathione (GSH), total chlorophyll, relative water content, Ca²⁺ and K⁺ concentrations and ascorbate, but there was a significant increase in H₂O₂, MDA, electron leakage (EL), proline, key antioxidant enzymes' activities, and endogenous Cd, NO and H₂S in the Cd-stressed plants. Silicon enhanced Cd tolerance of the pepper plants by lowering the leaf Cd concentration, oxidative stress, enhancing the antioxidant defence system, leaf Si content, photosynthetic traits and plant growth as well as the contents of NO, proline and H₂S. Furthermore, foliar-applied NO scavenger, cPTIO, and that of H₂S, hypotaurine (HT), significantly decreased the levels of H₂S alone, but cPTIO effectively reduced the concentrations of NO and H₂S accumulated by Si in the Cd-stressed plants. The positive effect of Si was eliminated by cPTIO, but not by HT, suggesting that both molecules were involved in Si-induced improvement in Cd tolerance of the pepper plants.

Keywords: Antioxidant system; Cadmium toxicity; Hydrogen sulfide; Nitric oxide; Pepper; Silicon.