In order to investigate molecular typing and fatty acid methyl esters (FAMEs) typing of clinical Stenotrophomonas maltophilia (S.maltophilia) isolates based on Random Amplification Polymorphic DNA (RAPD) and Gas Chromatography-Mass Spectrometer (GC-MS) methods, we collected 35 drug-resistant S. maltophilia isolates from March to December 2017 in a comprehensive hospital. The VITEK 2 Compact System was used to determine bacterial antibiotic susceptibility. The analysis of molecular typing was performed by RAPD. GC-MS was used to obtain FAMEs profiles. In total, all 35 isolates were multidrug-resistant S.maltophilia. Their resistance rates to CAZ and LEV were 21.4% and 21.1%, and to SXT up to 13.5%. S. maltophilia isolates were typed to six main clones by RAPD methods and four main clones by FAMEs fingerprint, respectively. The concordance rate of these two methods was 69.0%. Clonal typing provides evidence that multidrug-resistant isolates are prevalent among wards in the hospital. FAMEs profiles may be an easy and sensitive method for bacteria classification. The effectiveness and feasibility of different typing methods should be comprehensively considered.
Keywords: GC-MS; RAPD; Stenotrophomonas maltophilia; classification; drug-resistance; fatty acid.