Calcareous organic matter coatings sequester siderophores in alkaline soils

Rene M Boiteau; Ravi Kukkadapu; John B Cliff; Chuck R Smallwood; Libor Kovarik; Mark G Wirth; Mark H Engelhard; Tamas Varga; Alice Dohnalkova; Daniel E Perea; Thomas Wietsma; James J Moran; Kirsten S Hofmockel

doi:10.1016/j.scitotenv.2020.138250

Calcareous organic matter coatings sequester siderophores in alkaline soils

Sci Total Environ. 2020 Jul 1:724:138250. doi: 10.1016/j.scitotenv.2020.138250. Epub 2020 Mar 26.

Affiliations

¹ Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99354, United States of America; College of Earth, Ocean, Atmospheric Sciences, Oregon State University, Corvallis, OR 97331, United States of America. Electronic address: rene.boiteau@oregonstate.edu.
² Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99354, United States of America. Electronic address: ravi.kukkadapu@pnnl.gov.
³ Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99354, United States of America.
⁴ Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99354, United States of America; Bioscience Division, Department of Molecular and Microbiology, Sandia National Laboratories, Albuquerque, NM, 87185, United States of America.
⁵ Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99354, United States of America; Ecology, Evolution and Organismal Biology Department, Iowa State University, Ames, IA 50010, United States of America.

PMID: 32303367
DOI: 10.1016/j.scitotenv.2020.138250

Abstract

Although most studies of organic matter (OM) stabilization in soils have focused on adsorption to aluminosilicate and iron-oxide minerals due to their strong interactions with organic nucleophiles, stabilization within alkaline soils has been empirically correlated with exchangeable Ca. Yet the extent of competing processes within natural soils remains unclear because of inadequate characterization of soil mineralogy and OM distribution within the soil in relation to minerals, particularly in C poor alkaline soils. In this study, we employed bulk and surface-sensitive spectroscopic methods including X-ray diffraction, ⁵⁷Fe-Mössbauer, and X-ray photoemission spectroscopy (XPS), and transmission electron microscopy (TEM) methods to investigate the minerology and soil organic C and N distribution on individual fine particles within an alkaline soil. Microscopy and XPS analyses demonstrated preferential sorption of Ca-containing OM onto surfaces of Fe-oxides and calcite. This result was unexpected given that the bulk combined amounts of quartz and Fe-containing feldspars of the soil constitute ~90% of total minerals and the surface atomic composition was largely Fe and Al (>10% combined) compared to Ca (4.2%). Soil sorption experiments were conducted with two siderophores, pyoverdine and enterobactin, to evaluate the adsorption of organic molecules with functional groups that strongly and preferentially bind Fe. A greater fraction of pyoverdine was adsorbed compared to enterobactin, which is smaller, less polar, and has a lower aqueous solubility. Using NanoSIMS to map the distribution of isotopically-labeled siderophores, we observed correlations with Ca and Fe, along with strong isotopic dilution with native C, indicating associations with OM coatings rather than with bare mineral surfaces. We propose a mechanism of adsorption by which organics aggregate within alkaline soils via cation bridging, favoring the stabilization of larger molecules with a greater number of nucleophilic functional groups.

Keywords: Adsorption; Alkaline soil; Nanoscale; Organic-mineral interactions; Siderophores.