Mitochondria changes and metabolome differences of bovine longissimus lumborum and psoas major during 24 h postmortem

Qianqian Yu; Xiaojing Tian; Lele Shao; Xingmin Li; Ruitong Dai

doi:10.1016/j.meatsci.2020.108112

Mitochondria changes and metabolome differences of bovine longissimus lumborum and psoas major during 24 h postmortem

Meat Sci. 2020 Aug:166:108112. doi: 10.1016/j.meatsci.2020.108112. Epub 2020 Mar 14.

Authors

Qianqian Yu¹, Xiaojing Tian¹, Lele Shao¹, Xingmin Li¹, Ruitong Dai²

Affiliations

¹ College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, PR China; Beijing Higher Institution Engineering Research Center of Animal Product, China Agricultural University, No. 17 Qinghua East Road, Haidian District, Beijing 100083, PR China.
² College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, PR China; Beijing Higher Institution Engineering Research Center of Animal Product, China Agricultural University, No. 17 Qinghua East Road, Haidian District, Beijing 100083, PR China. Electronic address: dairuitong@hotmail.com.

PMID: 32302932
DOI: 10.1016/j.meatsci.2020.108112

Abstract

This study was performed to reveal mitochondria changes and metabolome differences between bovine longissimus lumborum (LL) and psoas major (PM) muscles within the first 24 h postmortem. PM showed significant lower (P < .05) pH than LL at 6 h and 12 h postmortem. LL had significant higher (P < .05) mitochondrial membrane potential and lower mitochondrial reactive oxygen species levels than PM at 24 h postmortem. For metabolome, distinctive separation between LL and PM could be seen from principal component analysis and hierarchical clustering analysis. Carnosine, L-histidine and adenosine diphosphate were found with higher abundances in LL. Whereas higher abundances of inosine and hypoxanthine were detected in PM, which indicated distinct purine metabolism rate between muscle types. Higher abundances of glycerophospholipids in PM may increase risk of oxidation damage, and subsequently result in inferior meat quality during extended retail display. The results of this study provided an idea for further research regarding the muscle-specific mitochondrial ROS on meat quality.

Keywords: Longissimus lumborum; Mitochondria; Psoas major; Untargeted metabolomics.

MeSH terms

Animals
Cattle
Membrane Potential, Mitochondrial
Metabolome*
Mitochondria / metabolism*
Muscle, Skeletal / metabolism
Reactive Oxygen Species / analysis
Red Meat / analysis*

Substances

Reactive Oxygen Species