Regulatory and Memory B Lymphocytes in Children With Newly Diagnosed Immune Thrombocytopenia

Asmaa M Zahran; Sanaa Shaker Aly; Ahmed Elabd; Ismail Lotfy Mohamad; Khalid I Elsayh

doi:10.14740/jh336w

Regulatory and Memory B Lymphocytes in Children With Newly Diagnosed Immune Thrombocytopenia

J Hematol. 2017 Oct;6(4):81-86. doi: 10.14740/jh336w. Epub 2017 Sep 20.

Authors

Asmaa M Zahran¹, Sanaa Shaker Aly², Ahmed Elabd³, Ismail Lotfy Mohamad⁴, Khalid I Elsayh⁴

Affiliations

¹ Clinical Pathology Department, South Egypt Cancer Institute, Assiut, Egypt.
² Clinical and Chemical Pathology Department, Faculty of Medicine, South Valley University, Qena, Egypt.
³ Pediatric Department, Faculty of Medicine, South Valley University, Qena, Egypt.
⁴ Pediatric Department, Faculty of Medicine, Assiut University, Assiut, Egypt.

PMID: 32300398
PMCID: PMC7155846
DOI: 10.14740/jh336w

Abstract

Background: Immune (idiopathic) thrombocytopenic purpura (ITP) is a primary autoimmune disease. It is characterized by a diminished peripheral platelet count (< 100 × 10⁹/L) caused by platelet destruction with an increased risk of mucocutaneous bleeding. The diagnosis of ITP depends on clinical characteristics and the laboratory examinations conducted, as well as the ability to exclude other diseases associated with thrombocytopenia. Antiplatelet autoantibodies are responsible for platelet destruction and probably for inhibition of megakaryopoiesis. B lymphocytes participate in immune responses through production of antibodies, antigen presentation to T cells, and cytokine secretion. The aims of this study were to investigate the levels of Bregs and memory B lymphocytes in newly diagnosed pediatric ITP patients and to correlate their levels with the course of the disease.

Methods: This study was a case-control study. The study included 30 patients with acute ITP. The patients were recruited from Pediatric Clinical Hematology Unit of Children Hospital, Assiut University. In addition, 20 healthy children of comparable age and sex were taken as controls. The institutional review board approved the study and informed consents were obtained.

Results: There is a significant alteration of B-cell homeostasis in patients with ITP.

Conclusion: Analysis of Bregs and memory B cells could serve as prognostic markers and might guide therapy in ITP patients in the future.

Keywords: B lymphocytes; Children; Immune; Thrombocytopenia.