miRNA-186 Improves Sepsis Induced Renal Injury Via PTEN/PI3K/AKT/P53 Pathway

Min Li; Wei Li; Feng-Qin Ren; Ming-Li Zhang

doi:10.1515/med-2020-0036

miRNA-186 Improves Sepsis Induced Renal Injury Via PTEN/PI3K/AKT/P53 Pathway

Open Med (Wars). 2020 Apr 4:15:254-260. doi: 10.1515/med-2020-0036. eCollection 2020.

Authors

Min Li¹, Wei Li¹, Feng-Qin Ren¹, Ming-Li Zhang¹

Affiliation

¹ Department of Intensive Care Unit, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong, 250013, China.

Abstract

Aim: The aim of this study is to explain the effects of miRNA-186 in renal injury induced by sepsis.

Methods: The Wistar rats were divided into 3 groups: the Sham group, Sepsis model group and the miRNA-186 group based on the model group; there were 9 rats in every group. The rat sepsis model was reproduced by cecal ligation and puncture (CLP). The rats of the miRNA-186 group were injected miRNA-186 from caudal vein. The rats of the difference group were killed after operation 24 h. The kidneys of the difference groups were taken for histopathological and cell apoptosis analysis by H&E and TUNEL assay. The relative protein expressions were measured by WB assay. miRNA-186 target to Phosphatase and tensin homologous protein (PTEN).

Results: Compared with the Sham group, the kidney histopathological and cell apoptosis rates of the model group were significantly damaged (P<0.05, respectively), however, the kidney histopathological and cell apoptosis rate of miRNA-186 group were significantly improved compared with the model group (P<0.05, respectively). The relative protein expressions were significantly different among 3 groups (P<0.05, respectively). The PTEN was the target of the miRNA-186.

Conclusion: miRNA-186 over-expression has effects that improve renal injury induced by sepsis via PTEN pathway.

Keywords: apoptosis; miRNA-186; renal injury.