In contrast to many biotic stresses that plants face, feeding by herbivores produces unique mechanical and chemical signatures. Plants have evolved effective systems to recognise these mechanical stimuli and chemical elicitors at the plasma membrane (PM), where this recognition generates ion fluxes, including an influx of Ca2+ that elicits cellular Ca2+ signalling, production of reactive oxygen species (ROS), and variation in transmembrane potential. These signalling events also function in propagation of long-distance signals (Ca2+ waves, ROS waves, and electrical signals), which contribute to rapid, systemic induction of defence responses. Recent studies have identified several candidate channels or transporters that likely produce these ion fluxes at the PM. Here, we describe the important roles of these channels/transporters in transduction or transmission of herbivory-induced early signalling events, long-distance signals, and jasmonic acid and green leaf volatile signalling in plants.