Common problems associated with the microbial productions of aromatic compounds and corresponding metabolic engineering strategies

Meijie Li; Changqing Liu; Jianming Yang; Rui Nian; Mo Xian; Fuli Li; Haibo Zhang

doi:10.1016/j.biotechadv.2020.107548

Common problems associated with the microbial productions of aromatic compounds and corresponding metabolic engineering strategies

Biotechnol Adv. 2020 Jul-Aug:41:107548. doi: 10.1016/j.biotechadv.2020.107548. Epub 2020 Apr 11.

Authors

Meijie Li¹, Changqing Liu², Jianming Yang³, Rui Nian⁴, Mo Xian⁵, Fuli Li⁶, Haibo Zhang⁷

Affiliations

¹ Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 135 Songling Road, Qingdao 266101, China; Energy-Rich Compound Production by Photosynthetic Carbon Fixation Research Center, College of Life Sciences, Qingdao Agricultural University, No. 700 Changchen Road, Qingdao 266109, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: li_mj@qibebt.ac.cn.
² Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 135 Songling Road, Qingdao 266101, China. Electronic address: liucq@qibebt.ac.cn.
³ Energy-Rich Compound Production by Photosynthetic Carbon Fixation Research Center, College of Life Sciences, Qingdao Agricultural University, No. 700 Changchen Road, Qingdao 266109, China.
⁴ Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 135 Songling Road, Qingdao 266101, China. Electronic address: nianrui@qibebt.ac.cn.
⁵ Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 135 Songling Road, Qingdao 266101, China. Electronic address: xianmo@qibebt.ac.cn.
⁶ Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 135 Songling Road, Qingdao 266101, China. Electronic address: lifl@qibebt.ac.cn.
⁷ Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 135 Songling Road, Qingdao 266101, China. Electronic address: zhanghb@qibebt.ac.cn.

PMID: 32289350
DOI: 10.1016/j.biotechadv.2020.107548

Abstract

Recent progress in metabolic engineering and synthetic biology has enabled the production of valuable chemicals in microbial cell factories from renewable feedstocks. High value-added aromatic compounds, most of which are traditionally chemically synthesized from petroleum-derived feedstocks, represent a large class of chemicals with industrial significance. The microbial biosynthesis of aromatic compounds has been studied for decades, and varying yields have been achieved for different aromatic compounds. In this review, we describe the common problems associated with the microbial biosynthesis of diverse aromatic compounds and summarize the corresponding metabolic engineering strategies for resolving these problems. In addition, future perspectives on the microbial production of aromatic compounds are discussed.

Keywords: Aromatic compounds; Cytotoxicity; Feedback inhibition; Metabolic engineering; PTS; Shikimate pathway.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Metabolic Engineering*
Organic Chemicals
Synthetic Biology*

Substances

Organic Chemicals