Homogeneous multiplexed digital detection of microRNA with ligation-rolling circle amplification

Chem Commun (Camb). 2020 May 19;56(40):5409-5412. doi: 10.1039/d0cc01530j.

Abstract

A simple multiplexed digital microRNA detection strategy with fluorescence flow cytometry was proposed. By isothermal ligation-rolling circle amplification, multiplexed microRNAs could be simultaneously converted to a series of nanoflower balls (NFBs) and counted by flow cytometry directly.

MeSH terms

  • DNA Probes / chemistry
  • DNA Probes / genetics
  • Flow Cytometry / methods*
  • Fluorescent Dyes / chemistry
  • Humans
  • Limit of Detection
  • MicroRNAs / analysis*
  • MicroRNAs / chemistry
  • MicroRNAs / genetics
  • Nanoparticles / chemistry
  • Nucleic Acid Amplification Techniques / methods
  • Nucleic Acid Hybridization
  • Proof of Concept Study

Substances

  • DNA Probes
  • Fluorescent Dyes
  • MIRN21 microRNA, human
  • MicroRNAs