Abstract
A simple multiplexed digital microRNA detection strategy with fluorescence flow cytometry was proposed. By isothermal ligation-rolling circle amplification, multiplexed microRNAs could be simultaneously converted to a series of nanoflower balls (NFBs) and counted by flow cytometry directly.
MeSH terms
-
DNA Probes / chemistry
-
DNA Probes / genetics
-
Flow Cytometry / methods*
-
Fluorescent Dyes / chemistry
-
Humans
-
Limit of Detection
-
MicroRNAs / analysis*
-
MicroRNAs / chemistry
-
MicroRNAs / genetics
-
Nanoparticles / chemistry
-
Nucleic Acid Amplification Techniques / methods
-
Nucleic Acid Hybridization
-
Proof of Concept Study
Substances
-
DNA Probes
-
Fluorescent Dyes
-
MIRN21 microRNA, human
-
MicroRNAs